- Primer sequences and amplicon size.
| Primers | Sequences | Band sizes (bp) | References |
|---|---|---|---|
| VacA (s)-F | 5’ATGGAAATACAACAAACACAC3’ | s1: 259, s2: 286 | 18,19 |
| VacA (s)-R | 5’CTGCTTGAATGCGCCAAAC3’ | ||
| VacA (m)-F | 5’CAATCTGTCCAATCAAGCGAG3’ | m1: 570, m2: 642 | |
| VacA (m)-R | 5’GCGTCTAAATAATTCCAAGG3’ | ||
| CagA-F | 5’AATACACCAACGCCTCCA3’ | 400 | |
| CagA-R | 5’TTGTTGCCGCTTTTGCTCTC3’ | ||
| 23s-F | ATGAATGGCGTAACGAGATG | 361 | 20 |
| 23s-R | GGAAATCGCAAGTTGAGTGT |
VacA: vacuolating cytotoxin A, CagA: cytotoxin associated antigen A