- The primer of genes involved in apoptosis and antiapoptotic genes.20-23
| Gene names | Primers sequence |
|---|---|
| Caspase-3 | F: 5′-GCTGGATGCCGTCTAGAGTC-3′ |
| R: 5′-ATGTGTGGATGATGCTGCCA-3′ | |
| Caspase-8 | F: 5′-AGAAGAGGGTCATCCTGGGAGA-3′ |
| R: 5′-TCAGGACTTCCTTCAAGGCTGC-3′ | |
| Caspase-9 | F: 5′-ATTGCACAGCACGTTCACAC-3′ |
| R: 5′-TATCCCATCCCAGGAAGGCA-3′ | |
| Bax | F: 5′-GAGCTAGGGTCAGAGGGTCA-3′ |
| R: 5′-CCCCGATTCATCTACCCTGC-3′ | |
| Bcl-2 | F: 5′-ACCTACCCAGCCTCCGTTAT-3′ |
| R: 5′-GAACTGGGGGAGGATTGTGG-3′ | |
| Bcl-XL | F: 5′-CAGAGCTTTGAACAGGTAG-3′ |
| R: 5′-GCTCTCGGGTGCTGTATTG-3′ | |
| p53 | F: 5′-GCTCTGACTGTACCACCATCC-3′ |
| F: 5′-CTCTCGGAACATCTCGAAGCG-3′ | |
| p21 | F: 5′-CTCAGAGGAGGCGCCATG-3′ |
| R: 5′-GGGCGGATTAGGGCTTCC-3′ | |
| GAPDH | F: 5′-CGGAGTCAACGGATTTGGTC-3′ |
| R: 5′-AGCCTTCTCCATGGTCGTGA-3′ | |
| Caspase: caspase protein, Bax: Bcl-2-associated X protein, Bcl-2: B-cell lymphoma 2, Bcl-XL: B-cell lymphoma-extra large, p53: Tumor protein P53, p21: Cyclin-dependent kinase inhibitor 1, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase | |