Table 1

- Primer pairs nucleotide sequences.

Genes	Oligonucleotide sequences (5′-3′)	Bp	Refs.
WNT-1^**	GCGTCTGATACGCCAAAATC	244	41
WNT-1^**	GGATTCGATGGAACCTTCTG	244	41
LRP-6^**	AGGCACTTACTTCCCTGCAA	273	42
LRP-6^**	GGGCACAGGTTCTGAATCAT	273	42
APC^**	CAAAGACTCTGTATTGTTGGGAAAT	98	^*
APC^**	CATCAGGCTGTGAGTGAATGA	98	^*
TCF-7^**	GGACAACTACGGGAAGAAGAAG	95	^*
TCF-7^**	TCTCCGGGTAAGTACCGAAT	95	^*
FZD-8^**	TCTTGTCGCTCACATGGTTC	375	^*
FZD-8^**	TGTAGAGCACGGTGAACAGG	375	^*
LEF-1^**	GAAGCCTCAGCATGAACAGA	110	^*
LEF-1^**	CAACGACATTCGCTCTCATTTC	110	^*
NOTCH-1^†	CAATGTGGATGCCGCAGTTGTG’	125	43
NOTCH-1^†	CAGCACCTTGGCGGTCTCGTA	125	43
JAG-1^†	GTATCTGTCCACCTGGCTATG	120	^*
JAG-1^†	ACAGACACTGGAATCTGTTGAT	120	^*
SHH^{^‡}	CCGAGCGATTTAAGGAACTCA	133	^*
SHH^{^‡}	ATCACCGAGATGGCCAAAG	133	^*
PTCH^‡	GGGTGGCACAGTCAAGAACAG	108	44
PTCH^‡	TACCCCTTGAAGTGCTCGTACA	108	44
SMO^{^‡}	ATCGCTACCCTGCTGTTATTC	90	^*
SMO^{^‡}	GGGCACCATCCATGAACT	90	^*
GLI-1^{^‡}	GAGCTGGACATGCTGGTT	148	^*
GLI-1^{^‡}	ATTCAGGCTCACGCTTCTC	148	^*
MMP-1^{^§}	GCCTTCCAACTCTGGAGTAATG	111	^*
MMP-1^{^§}	AGGAGAGTTGTCCCGATGAT	111	^*
SOX^{^§}	GCCGAGTGGAAACTTTTGTCG	150	^*
SOX^{^§}	GGCAGCCTGTACTTATCCTTC	150	^*
NANOG^††	TTTGTGGGCCTGAAGAAAACT	116	^*
NANOG^††	AGGGCTGTCCTGAATAAGCAG	116	^*
OCT4^{^††}	CAAGGAGGCAAAGGTCTCGAA	193	^*
OCT4^{^††}	CGGCACCTGACACAGAAGA	193	^*
GAPDH^{^‡‡}	GGAGCGAGATCCCTCCAAAAT	197	^*
GAPDH^{^‡‡}	GGCTGTTGTCATACTTCTCAT	197	^*
CYPA^‡‡	TATCTGCACTGCCAAGACTGAGTG	124	^*
CYPA^‡‡	CTTCTTGCTGCTGGTCTTGCATTCC	124	^*

Bp: base pair, Refs.: references

↵* Designed in this study.
↵** Wnt,
↵^† Notch,
↵^‡ Hedgehog,
↵^§ metastasis,
↵^†† stem cell markers, and
↵^‡‡ housekeeping genes.