- Histological feature analysis of the 56 suspected mycosis fungoides (MF) lesions involved in the study.
| Case no. | Diagnosis | Clinical feature | Epidermotropism | Epidermotropism grade * | Pautrier’s microabscess | Spongiosis | Spongiosis grade | Parakeratosis |
|---|---|---|---|---|---|---|---|---|
| 1 | MF | patch | 1 | 1 | 1 | 1 | 1 | 1 |
| 2 | MF | patch | 1 | 1 | 1 | 1 | 1 | 1 |
| 3 | MF | patch | 1 | 1 | 0 | 1 | 1 | 1 |
| 4 | MF | patch | 1 | 1 | 0 | 1 | 1 | 1 |
| 5 | MF | patch | 1 | 1 | 0 | 1 | 1 | 0 |
| 6 | MF | patch | 1 | 1 | 0 | 1 | 1 | 0 |
| 7 | MF | patch | 1 | 2 | 0 | 1 | 1 | 1 |
| 8 | MF | patch | 1 | 2 | 1 | 1 | 1 | 1 |
| 9 | MF | patch | 0 | 0 | 0 | 1 | 1 | 0 |
| 10 | MF | patch | 0 | 0 | 0 | 1 | 1 | 1 |
| 11 | MF | patch | 0 | 0 | 0 | 1 | 1 | 1 |
| 12 | MF | plaque | 0 | 0 | 1 | 1 | 2 | 1 |
| 13 | MF | plaque | 1 | 1 | 1 | 1 | 1 | 1 |
| 14 | MF | plaque | 1 | 1 | 1 | 1 | 1 | 1 |
| 15 | MF | plaque | 1 | 1 | 1 | 1 | 1 | 1 |
| 16 | MF | plaque | 1 | 1 | 1 | 1 | 1 | 1 |
| 17 | MF | plaque | 1 | 1 | 1 | 1 | 1 | 1 |
| 18 | MF | plaque | 1 | 1 | 0 | 1 | 1 | 0 |
| 19 | MF | plaque | 1 | 1 | 0 | 1 | 1 | 0 |
| 20 | MF | plaque | 1 | 1 | 0 | 1 | 1 | 0 |
| 21 | MF | plaque | 1 | 2 | 1 | 1 | 1 | 1 |
| 22 | MF | plaque | 1 | 2 | 1 | 1 | 1 | 1 |
| 23 | MF | plaque | 1 | 2 | 1 | 1 | 2 | 1 |
| 24 | MF | plaque | 1 | 1 | 0 | 1 | 1 | 1 |
| 25 | MF | plaque | 1 | 1 | 1 | 0 | 0 | 1 |
| 26 | MF | nodule | 1 | 1 | 1 | 1 | 1 | 1 |
| 27 | MF | nodule | 1 | 1 | 1 | 0 | 0 | 0 |
| 28 | MF | nodule | 0 | 0 | 0 | 1 | 1 | 0 |
| 29 | MF | nodule | 0 | 0 | 0 | 0 | 0 | 0 |
| 30 | MF | erythroderma | 1 | 1 | 0 | 1 | 1 | 1 |
| 31 | MF | erythroderma | 0 | 0 | 0 | 1 | 1 | 1 |
| 32 | MF | poikiloderma | 1 | 1 | 0 | 1 | 1 | 0 |
| 33 | MF | poikiloderma | 0 | 0 | 0 | 0 | 0 | 0 |
| 34 | MF | follicular | 1 | 1 | 1 | 1 | 1 | 0 |
| 35 | non-MF | patch | 0 | 0 | 0 | 0 | 0 | 0 |
| 36 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 1 |
| 37 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 1 |
| 38 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 0 |
| 39 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 0 |
| 40 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 0 |
| 41 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 0 |
| 42 | non-MF | patch | 0 | 0 | 0 | 1 | 1 | 0 |
| 43 | non-MF | patch | 0 | 0 | 0 | 1 | 2 | 0 |
| 44 | non-MF | plaque | 0 | 0 | 0 | 1 | 1 | 1 |
| 45 | non-MF | plaque | 0 | 0 | 0 | 1 | 1 | 1 |
| 46 | non-MF | plaque | 0 | 0 | 0 | 1 | 1 | 1 |
| 47 | non-MF | plaque | 0 | 0 | 0 | 1 | 1 | 0 |
| 48 | non-MF | plaque | 0 | 0 | 0 | 1 | 1 | 0 |
| 49 | non-MF | plaque | 0 | 0 | 0 | 1 | 2 | 0 |
| 50 | non-MF | plaque | 0 | 0 | 0 | 0 | 0 | 0 |
| 51 | non-MF | plaque | 0 | 0 | 0 | 1 | 1 | 0 |
| 52 | non-MF | follicular | 0 | 0 | 0 | 1 | 1 | 0 |
| 53 | non-MF | macule | 0 | 0 | 0 | 0 | 0 | 0 |
| 54 | non-MF | macule | 0 | 0 | 0 | 0 | 0 | 0 |
| 55 | non-MF | macule | 0 | 0 | 0 | 0 | 0 | 0 |
| 56 | non-MF | macule | 0 | 0 | 0 | 0 | 0 | 0 |
| Case no. | Parakeratosis grade | Atypical lymphocytes | Atypical lymphocytes grade | Cerebriform lymphocytes | Cerebriform lymphocytes grade | Infiltrating lymphocytes | Infiltration grade | Pigment incontinence | Adnexal involvement | Dermal fibrosis |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 |
| 2 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 1 |
| 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 |
| 4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 |
| 5 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
| 6 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 7 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 1 |
| 8 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 1 |
| 9 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 |
| 10 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 11 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 12 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 13 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 14 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 15 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 16 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 17 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 |
| 18 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
| 19 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 |
| 20 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 |
| 21 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 22 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 |
| 23 | 2 | 1 | 2 | 1 | 3 | 1 | 2 | 0 | 0 | 1 |
| 24 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
| 25 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 |
| 26 | 1 | 1 | 2 | 1 | 2 | 1 | 3 | 1 | 1 | 1 |
| 27 | 0 | 1 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 1 |
| 28 | 0 | 1 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 |
| 29 | 0 | 1 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 |
| 30 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 1 |
| 31 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 1 |
| 32 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 |
| 33 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 |
| 34 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 1 |
| 35 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 36 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 |
| 37 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 |
| 38 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 39 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 40 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 41 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 42 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 43 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 44 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 45 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 |
| 46 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 |
| 47 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 48 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 49 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 50 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 51 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 52 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 53 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 54 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 55 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
| 56 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
↵* Traits were graded from 0 to 3 in epidermotropism, spongiosis, parakeratosis, dermal infiltrate, atypical lymphocytes, and large atypical cerebriform cells and graded from 0 to 1 in Pautrier’s microabscess, pigment incontinence, dermal fibrosis, and adnexal involvement. Analysis was performed using Fisher’s exact test with a Bonferroni-corrected threshold of significance = 0.005 = 0.05/10). If a significant association found, follow-up testing was performed to assess the grade severity of a trait vis-à-vis the diagnosis, using ordinal logistic regression (using maximum likelihood fitting), in 2 ways: first, by including zero values in the response variable (0, 1, 2, 3; 5 models fitted), and second, by considering only non-zero cases (such as including only those patients exhibiting the trait). When narrowing the testing to the patch and plaque groups only, 2×2 contingency tables (Fisher’s exact test, 2-tailed) were used. Likewise, it is pertinent to know whether the grade severity of a trait differentiating MF from a non-MF diagnosis was also more pronounced in patch versus plaque patients. To this end, non-zero cases were excised, and contingency tables were analysed (9 2×2 tables with Fisher’s exact test and one 3×2 table analysed with a likelihood ratio test of the chi-square). Finally, for either the patch or plaque subgroup, 10 goodness-of-fit tests were performed, 1 per histological trait, to determine where the observed counts of presence/absence deviated from those expected by chance (such as 50% frequency of occurrence).