Table 2

- Polymerase chain reaction conditions for multiplex polymerase chain reaction used for Y-chromosome microdeletion-assay.

Step 1	Step 2 (4 cycles)			Step 3 (3 cycles)			Step 4 (3 cycles)			Step 4 (25 cycles)			Step 5 (one cycle)
94°C	94°C	61°C	72°C	94°C	59°C	72°C	94°C	57°C	72°C	94°C	55°C	72°C	72°C
2 min	one min	30 sec	one min	one min	30 sec	one min	one min	30 sec	one min	one min	30 sec	one min	7 min

°C: degrees Celieus, Min: minute, Sec: second