Skip to main content
Log in

Frequency of HLA antibodies in south India

  • Published:
Journal of Biosciences Aims and scope Submit manuscript

Abstract

Sera from 4088 pregnant women (649 antenatal bleeding and 3439 post-partum bleeding) living in Madurai, were collected and screened for anti-HLA. A, B and DR antibodies. 696 of them were screened for anti-HLA DR antibodies. Ten per cent (65/649) of antenatal sera and 13 4% of post-partum sera (463/3439) were positive for HLA A and B antibodies: nonetheless the percentage of monospecific sera were almost the same in both. Screening for HLA DR antibodies were carried out using platelet absorption in test tray technique: seventy three of 696 (10.5%) were positive. The incidence of anti-HLA A, B antibodies correlates to the allelic frequencies in the population. Thus in India, collection and screening post-partum haemorrhage is the simplest and cost effective method of acquiring polyclonal sera for routine laboratory and diagnostic use.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bodmer J G, Marsh S G E, Albert E D, Bodmer W F, Dupont B, Erlich H A, Mach B, Mayr W R, Parham P, Sasazuki T, Schreuder G M Th, Strominger J L, Svejgaard A and Terasaki P I 1992 Nomenclature for factors of the HLA system, 1991;Tissue Antigens 39 161–173

    Article  CAS  Google Scholar 

  • Boyum A 1968 Separation of Leukocytes from Blood and Bone marrow;Scand. J. Clin. Lab. Invest,21 97

    Article  Google Scholar 

  • Brahmajothi V, Pitchappan R M, Kakkanaiah V N, Sasidhar M, Rajaram K, Ramu S, Palanimurugan K, Paramasivam C N and Prabhakar R 1991 Association of Pulmonary tuberculosis and HLA in South India;Tubercle 72 123–132

    Article  CAS  Google Scholar 

  • Dausset J 1958 Iso-leuco-anticorps;Acta Haematol. 20 156

    Article  CAS  Google Scholar 

  • Decary F, van Helden-Henningheim L, van Griethuysen D, Helmerhost F M, van der Werf A J M and Engelfriet C P 1979 Detection of Bcell specific alloantibodies in pregnancy sera in the lymphocytotoxicity and the indirect immunofluorescence techniques;Tissue Antigens 14 1

    Article  CAS  Google Scholar 

  • Manickasundari M, Selvaraj P and Pitchappan R M 1984 Studies on T cells of the lizard calotes versicolor: Nylon wool adherent and non-adherent population of spleen;Dev. Comp. Immunol. 8 364–374

    Article  Google Scholar 

  • Nymand G, Heron I, Jensen G and Lundsgaard A 1971 Occurance of cytotoxic antibodies during pregnancy;Vox Sang. 2121

  • Payne R and Rolfs M R 1958 Fetomaternal leukocyte incompatibility;J. Clin. Invest. 37 1756

    Article  CAS  Google Scholar 

  • Pitchappan R M and Arulraj N 1989 A versatile computer Data-base for Human ImmunogeneticStudiesIndian J.Physiol. Anthropol. HumanGenent. 15 81–92

    Google Scholar 

  • Regan L, Braude P R and Hill DP 1991 A prospective study of the incidence, time of appearance and significance of anti-paternal lymphocytotoxic antibodies in human pregnancy;Hum. Reprod. 6 294–298

    Article  CAS  Google Scholar 

  • Rodey G E, Anderson J and Aster R H 1979 Acquistion of HLA lymphocytotoxic antibodies;NIAID Manualof Tissue Typing Techniques,NIH Publications No. 80-545 186

  • Simonney N, Laynisse Z, Balbas O, Garcia E and Stoikow Z 1984 Cytotoxic antibodies in sera of Venezuelan multiparous women of Amerindian and mixed ethnic origin;Tissue Antigens 23 117–126

    Article  CAS  Google Scholar 

  • Snedecor G W and Cochran W G 1968Statistical methods, (Iowa: Iowa State University Press)

    Google Scholar 

  • Terasaki P I and McClelland J D 1964 Microdroplet assay of human serum cytotoxin;Nature (London) 204 998–999

    Article  CAS  Google Scholar 

  • Terasaki P I, Mickey M R, Yamosaki J N and Vredevoc D L 1970 Maternal-fetal imcompatibility;I. Transplant. 9 538

    Article  CAS  Google Scholar 

  • Thomsen A C, Sommer E, Demur C and Calot M and Ohayou E 1985 Platlet absorption on the test tray (PATT): A rapid method for the screening of HLA Class II antibodies using the two colour fluorescence method;Tissue Antigens 26 193–200

    Article  Google Scholar 

  • Trowsdale J, Ragoussis J and Campbell R D 1991 Map of the human MHC;Immunol. Today 12 443–446

    Article  CAS  Google Scholar 

  • Van Rood J J, Eernisse J G and Leeuwen A 1958 Leukocyte antibodies in sera from pregnant women;Nature (London) 181 1735–1736

    Article  Google Scholar 

  • Van Rood J J and Van Leeuwen A 1980 The serology of HLA-DR;Exp. Clin. Immunol. 4 113–122

    Google Scholar 

  • Vives J, Gelabert A and Castillo R 1976 HLA antibodies and period of Gestation: Decline in frequency of positive sera during last trimester;Tissue Antigens 7 209

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pitchappan, R.M., Amutha, S., Mahendran, V. et al. Frequency of HLA antibodies in south India. J. Biosci. 18, 373–380 (1993). https://doi.org/10.1007/BF02702995

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02702995

Keywords

Navigation