Original articleThickness Mapping of Retinal Layers by Spectral-Domain Optical Coherence Tomography
Section snippets
Materials and Methods
SD-OCT imaging was performed in 1 eye of 15 normal subjects, 8 female and 7 male, 8 right and 7 left eyes. The subjects' ages ranged between 40 and 59 years, with an average age of 52 ± 6 years (mean ± standard deviation).
SD-OCT imaging was performed using a commercially available OCT instrument (Spectralis, Heidelberg Engineering, Heidelberg, Germany). Nineteen horizontal SD-OCT B-scans were acquired in each eye, encompassing a 6 × 5-mm retinal area, centered on the fovea. Each SD-OCT image
Results
By automated segmentation of 19 SD-OCT images, thickness maps were generated in 6 retinal layers: nerve fiber layer (layer 1), ganglion cell layer + inner plexiform layer (layer 2), inner nuclear layer (layer 3), outer plexiform layer (layer 4), outer nuclear layer + photoreceptor inner segments (layer 5), and photoreceptor outer segments (layer 6). Examples of thickness maps generated from the right eye of 1 subject are shown in Figure 3. The thickness map of layer 1 (nerve fiber layer)
Discussion
Thickness mapping of retinal layers can be useful for detection and monitoring of thickness alterations in specific retinal layers associated with retinal pathologies. In the current study, application of a novel automated image segmentation technique for thickness mapping of 6 retinal layers using SD-OCT technology was reported. Thickness maps generated in normal subjects corresponded with normal retinal anatomy. Total retinal thickness derived by automated thickness mapping highly correlated
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