RadiobiologyDown-regulation of survivin by oxaliplatin diminishes radioresistance of head and neck squamous carcinoma cells
Section snippets
Cell culture
Two HNSCC (NT8e, Cal27) and one normal 293-cell line were maintained in culture media (DMEM) with 2 mM glutamine, 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin as per standard culture conditions. Two cell lines (NT8e and 293) were provided by Dr. Mulherkar as a gift and Cal27 cell line was obtained from American Type Culture Collection (Manassas, VA). NT8e and Cal27, shown to resist conventional cancer therapies, have primarily been isolated from upper
Radiation treatment induced survivin expression
Both cancer cell lines were highly expressive for survivin but normal 293-cell line was found negative for survivin expression (Fig. 1A). Further we examined the effect of radiation treatment on survivin expression in our selected cell lines. Radiation treatment significantly induced the expression of survivin in cancer cell lines at both doses (3 and 6 Gy) and time points (12 and 24 h) whereas no effect was detected in 293-cell line (Fig. 1B).
Over-expression of survivin provides resistance to radiation
To elucidate whether survivin is directly involved in
Discussion
Radiotherapy and surgery are the cornerstones in treating head and neck cancer [23]. Locoregional recurrence remains the major pattern of treatment failure. About 40% of patients experienced locoregional tumor progression within five years after therapy [24]. Also resistance hampers the long-term effectiveness of radiotherapy in HNSCC. Thus understanding of the molecular mechanisms contributing to drug resistance may provide opportunity to develop more effective anti-cancer therapeutic
Acknowledgment
The authors are thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi for the award of Emeritus Scientist to Prof. P.S. Bisen and to Dr. Rita Mulherkar, ACRTEC, Tata Memorial Centre, Navi Mumbai for providing cell lines for this study.
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