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Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B

Leukemia volume 17, pages 1605–1612 (2003)Cite this article

Abstract

B-cell-specific plasma-membrane proteins are potential targets for either small molecule or antibody-based therapies. We have sought to annotate proteins expressed at the cell surface membrane in patients with chronic lymphocytic leukemia (CLL) using plasma-membrane-based proteomic analysis to identify previously uncharacterized and potentially B-cell-specific proteins. Proteins from plasma-membrane fractions were separated on one-dimensional gels and trypsinized fractions subjected to high-throughput MALDI-TOF mass spectrometry. Using this method, many known B-cell surface antigens were detected, but also known proteins not previously described in this disease or in this cellular compartment, including cell surface receptors, membrane-associated enzymes and secreted proteins, and completely unknown proteins. To validate the method, we show that BLK, a B-cell-specific kinase, is located in the CLL-plasma-membrane fraction. We also describe two novel proteins (MIG2B and B-cell novel protein #1, BCNP1), which are expressed preferentially in B cells. MIG2B is in a highly conserved and defined gene family containing two plasma-membrane-binding ezrin/radixin/moesin domains and a pleckstrin homology domain; the Caenorhabditis elegans homolog (UNC-112) is a membrane-associated protein that colocalizes with integrin at cell–matrix adhesion complexes. BCNP1 is a completely unknown protein with three predicted transmembrane domains, with three alternatively spliced final exons. Proteomic analysis may thus define new potential therapeutic targets.

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Acknowledgements

This work was supported in part by grants from the Kay Kendall Leukemia Fund and the Leukemia Research Fund.

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Author notes

  1. R S Boyd and P J Adam: These authors contributed equally to the work presented in this paper

Authors and Affiliations

  1. Oxford Glycosciences (UK) Ltd, Abingdon, Oxford, UK

    R S Boyd, P J Adam, S Patel, J A Loader, J Berry, N T Redpath, H R Poyser, G C Fletcher, N A Burgess, A C Stamps, L Hudson, P Smith, M Griffiths & J A Terrett

  2. Academic Haematology, Institute of Cancer Research, Sutton, Surrey, UK

    T G Willis, E L Karran, D Catovsky & M J S Dyer

  3. Department of Haematology, MRC Toxicology Unit/University of Leicester, UK

    E L Karran & M J S Dyer

  4. Department of Haematology, Royal Bournemouth Hospital, UK

    D G Oscier

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  1. R S Boyd
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  11. L Hudson
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  18. J A Terrett
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Boyd, R., Adam, P., Patel, S. et al. Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B. Leukemia 17, 1605–1612 (2003). https://doi.org/10.1038/sj.leu.2402993

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