Abstract
A chromosomal gene (mdrL) was found in Listeria monocytogenes L028, showing a high degree of similarity with multidrug efflux transporters of the major facilitator superfamily (family 2). An allele-substituted mutant of this gene failed to pump out ethidium bromide and presented lower minimal inhibitory concentrations of macrolides, cefotaxime and heavy metals. This is the first multidrug efflux pump described in Listeria.
MeSH terms
-
ATP Binding Cassette Transporter, Subfamily B / chemistry*
-
ATP Binding Cassette Transporter, Subfamily B / genetics
-
ATP Binding Cassette Transporter, Subfamily B / metabolism*
-
Amino Acid Motifs
-
Amino Acid Sequence
-
Anti-Bacterial Agents / pharmacology
-
Bacterial Proteins / chemistry*
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism*
-
Drug Resistance, Microbial / genetics
-
Drug Resistance, Multiple / genetics
-
Ethidium / metabolism
-
Listeria monocytogenes / drug effects*
-
Listeria monocytogenes / genetics
-
Listeria monocytogenes / metabolism
-
Metals, Heavy / pharmacology
-
Microbial Sensitivity Tests
-
Molecular Sequence Data
-
Mutation
Substances
-
ATP Binding Cassette Transporter, Subfamily B
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
Metals, Heavy
-
Ethidium