Point-of-care (POC) assays for autoantibodies to thyroid peroxidase (TPOAb) and to thyroglobulin (TgAb) are described. Both assays are based on the ability of autoantibodies in test samples (whole blood, plasma, or sera) to inhibit the binding of monoclonal antibodies to TPO or to Tg. The assays require no special equipment and give results in 10 minutes. Analysis of samples from healthy blood donors (n = 80), patients with autoimmune thyroid disease (n = 97) and nonthyroid autoimmune diseases (n = 20) showed that results with the POC tests compared well to those obtained by agglutination assay and enzyme-linked immunosorbent assay (ELISA). The reference immunoprecipitation assays (IPA) based on 125I-labeled TPO or Tg were more sensitive than the POC tests particularly in the case of TgAb measurements. However, no samples were found positive by POC test and negative by IPA emphasizing the high specificity of the POC assays. Our results suggest that POC testing for TPOAb and TgAb with assays such as those we describe could be useful in certain situations. These include prediction of postpartum thyroiditis and the development of interferon-alpha-related thyroid disease.