Abstract
To demonstrate the effect of zoledronic acid in proliferation, invasion, and migration of human nasopharyngeal carcinoma cell HNE-1 and explore the potential role of VEGF, MMP-2, and MMP-9 proteins in vitro. Human nasopharyngeal carcinoma cell HNE-1 was exposed to various concentrations (0-40 μmol/l) of zoledronic acid. Zoledronic acid inhibited proliferation of HNE-1 cells though not in a dose-dependent manner. Zoledronic acid had exerted a dose-dependent effect on the migration and invasion of HNE-1 cells. Both expressions of mRNA and protein of MMP2, MMP9, and VEGF were reduced, respectively, detected by RT-PCR and Western blot assays. These data suggested that zoledronic acid not only inhibited growth but also invasion and migration of HNE-1 cells in vitro. The anti-cancer action of zoledronic acid was partially associated with the suppression of VEGF expression and secretion and downregulating the expression of MMP2 and MMP9.
MeSH terms
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Blotting, Western
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Bone Density Conservation Agents / pharmacology*
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Carcinoma
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Cell Adhesion / drug effects
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Cell Movement / drug effects*
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Cell Proliferation / drug effects*
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Diphosphonates / pharmacology*
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Down-Regulation
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Enzyme-Linked Immunosorbent Assay
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Humans
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Imidazoles / pharmacology*
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Matrix Metalloproteinase 2 / genetics*
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Matrix Metalloproteinase 2 / metabolism
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Matrix Metalloproteinase 9 / genetics*
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Matrix Metalloproteinase 9 / metabolism
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Nasopharyngeal Carcinoma
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Nasopharyngeal Neoplasms / genetics
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Nasopharyngeal Neoplasms / metabolism*
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Nasopharyngeal Neoplasms / pathology*
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Neoplasm Invasiveness
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Cells, Cultured
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Vascular Endothelial Growth Factor A / genetics*
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Vascular Endothelial Growth Factor A / metabolism
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Zoledronic Acid
Substances
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Bone Density Conservation Agents
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Diphosphonates
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Imidazoles
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RNA, Messenger
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VEGFA protein, human
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Vascular Endothelial Growth Factor A
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Zoledronic Acid
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MMP2 protein, human
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9