Detection of Toxoplasma gondii and Cyclospora cayetanensis in Mytilus galloprovincialis from Izmir Province coast (Turkey) by Real Time PCR/High-Resolution Melting analysis (HRM)

Food Microbiol. 2014 Dec:44:128-35. doi: 10.1016/j.fm.2014.05.012. Epub 2014 Jun 2.

Abstract

To evaluate the presence of Toxoplasma gondii and Cyclospora cayetanensis in edible shellfish, a total of 795 specimens of Mytilus galloprovincialis from 8 different sites on the west coast of Turkey (Bays of Izmir and Mersin) were examined. Fifty-three pooled samples were created and subjected to EvaGreen(®) RealTime PCR assay and HRM analysis for protozoans detection and oocyst quantification. Seven out of 8 sites were contaminated with at least one protozoan species. Twenty-one out of 53 (39.6%) samples tested positive for T. gondii, C. cayetanensis or both pathogens: 26.4% samples positive for C. cayetanensis; 9.4% for T. gondii, and 3.8% for both protozoans. The number of oocysts in positive samples ranged from 6 to 30 for T. gondii and from 3 to 48 for C. cayetanensis. This is the first report of T. gondii Type 1, and C. cayetanensis in M. galloprovincialis, the most consumed shellfish species in Turkey.

Keywords: Cyclospora cayetanensis; Mytilus galloprovincialis; Public health; RealTime HRM; Toxoplasma gondii Type I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclospora / chemistry
  • Cyclospora / genetics
  • Cyclospora / isolation & purification*
  • DNA, Protozoan / adverse effects
  • DNA, Protozoan / chemistry
  • Food Contamination / analysis*
  • Mytilus / parasitology*
  • Real-Time Polymerase Chain Reaction
  • Shellfish / parasitology*
  • Toxoplasma / chemistry
  • Toxoplasma / genetics
  • Toxoplasma / isolation & purification*
  • Transition Temperature
  • Turkey

Substances

  • DNA, Protozoan