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Research ArticleOriginal Article
Open Access

Investigation of cryptosporidiosis by enzyme-linked immunosorbent assay and microscopy in children with diarrhea

Hasan Yilmaz, Zeynep Tas-Cengiz and Mutalip Cicek
Saudi Medical Journal April 2008, 29 (4) 526-529;
Hasan Yilmaz
Department of Parasitology, Yuzuncu Yil University Faculty of Medicine, Van, 65200, Turkey. Tel. +90 (432) 2150470. Fax. +90 (432) 2167519. E-mail: [email protected]
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Zeynep Tas-Cengiz
Department of Parasitology, Yuzuncu Yil University Faculty of Medicine, Van, 65200, Turkey. Tel. +90 (432) 2150470. Fax. +90 (432) 2167519. E-mail: [email protected]
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Mutalip Cicek
Department of Parasitology, Yuzuncu Yil University Faculty of Medicine, Van, 65200, Turkey. Tel. +90 (432) 2150470. Fax. +90 (432) 2167519. E-mail: [email protected]
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Abstract

OBJECTIVE: To investigate the frequency of cryptosporidiosis by enzyme-linked immunosorbent assay ELISA and microscopy and its relationship with diarrhea.

METHODS: The study was prospectively performed in the Parasitology Laboratory and Pediatric Outpatient Clinic of the Research Hospital, Yuzuncu Yil University, Van, Turkey between 2004 and 2006. Stool samples were obtained from a total of 2000 children with diarrhea, 870 females, and 1130 males aging 0-15 years as study group, and 100 children of the same age were randomly selected as a control group. The flotation method was firstly carried out for all stool samples in saturated zinc sulfate solution, then staining process by modified acid-fast staining. All samples were also tested for Cryptosporidium parvum antigen by ELISA. Native-Lugol and trichrome staining were used to identify other intestinal parasites.

RESULTS: The antigen was determined in 97 4.9% of 2000 children by ELISA, however, the oocysts were only seen in 39 children 1.95% by microscopy. Cryptosporidium spp. were not detected in the control group either by ELISA or by microscopy. We found a significant p<0.001 relationship between diarrhea and cryptosporidiosis. Other intestinal parasites were detected in 713 35.7% of 2000 diarrheic children.

CONCLUSION: Cryptosporidium spp. antigen searching by ELISA in stool samples should be included for diagnosis of the disease in all hospitals.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 29 (4)
Saudi Medical Journal
Vol. 29, Issue 4
1 Apr 2008
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Investigation of cryptosporidiosis by enzyme-linked immunosorbent assay and microscopy in children with diarrhea
Hasan Yilmaz, Zeynep Tas-Cengiz, Mutalip Cicek
Saudi Medical Journal Apr 2008, 29 (4) 526-529;

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Investigation of cryptosporidiosis by enzyme-linked immunosorbent assay and microscopy in children with diarrhea
Hasan Yilmaz, Zeynep Tas-Cengiz, Mutalip Cicek
Saudi Medical Journal Apr 2008, 29 (4) 526-529;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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