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Research ArticleOriginal Article
Open Access

Isolation and cloning of human papillomavirus 16 L1 gene from Iranian isolate

Ali Teimoori, Hoorieh Soleimanjahi, Fatemeh Fotouhi and Zahra Meshkat
Saudi Medical Journal August 2008, 29 (8) 1105-1108;
Ali Teimoori
Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
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Hoorieh Soleimanjahi
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Fatemeh Fotouhi
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Zahra Meshkat
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Abstract

OBJECTIVE: To isolate and construct cloning and expression vectors containing human papillomavirus (HPV16-L1) gene as target for application as recombinant vaccine.

METHODS: The study was performed in 2007 in Tarbiat Modares University, Tehran, Iran. Four genital specimens DNAs were amplified with the use of HPV type-specific primers based on HPV-16 L1, E6, and E7 genes. The polymerase chain reaction products were cloned into suitable cloning and expression vectors and were confirmed by restriction enzyme analysis and sequencing.

RESULTS: The desired plasmids were sequenced and indicated 99% homology with those submitted full length L1 sequences in the GenBank.

CONCLUSION: The results showed that there was 99% homology between our product and those mentioned in the GenBank. Nowadays empty viral capsids, termed virus-like particles, are synthesized with the use of microbial or cellular expression systems. Therefore, it can be concluded that the Iranian HPV16 full length L1 sequence is very similar to the other sequences in the GenBank and it can be used as a candidate gene in prophylactic vaccine for cervical cancer.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 29 (8)
Saudi Medical Journal
Vol. 29, Issue 8
1 Aug 2008
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Isolation and cloning of human papillomavirus 16 L1 gene from Iranian isolate
Ali Teimoori, Hoorieh Soleimanjahi, Fatemeh Fotouhi, Zahra Meshkat
Saudi Medical Journal Aug 2008, 29 (8) 1105-1108;

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Isolation and cloning of human papillomavirus 16 L1 gene from Iranian isolate
Ali Teimoori, Hoorieh Soleimanjahi, Fatemeh Fotouhi, Zahra Meshkat
Saudi Medical Journal Aug 2008, 29 (8) 1105-1108;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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