Oxidative stress in recurrent pregnancy loss women.

OBJECTIVES: To investigate biochemical changes in lipid peroxidation, nitric oxide, and vitamin E in recurrent pregnancy loss women, and compare these with healthy pregnant, and non-pregnant women.

METHODS: A case control study was conducted from September 2008 to December 2009 at Al-Khadimiya Teaching Hospital, Baghdad, Iraq. Ninety-six subjects were included in the study, 32 were patients with recurrent pregnancy loss (RPL), and 32 pregnant women in their third trimester, and another 32 non-pregnant women were used as controls. Blood samples were collected from each patient at the time of pregnancy loss. Serum from patients and controls was then used to estimate malondialdehyde (MDA), nitric oxide (NO), and vitamin E levels.

RESULTS: There was a significant elevation in patient serum MDA compared with third trimester pregnant women (p=0.002) and non-pregnant women (p=0.0001). Both serum vitamin E and NO levels in RPL patients also showed a highly significant decrease compared with third trimester pregnant, and non-pregnant women. A highly significant difference was found in the MDA/vitamin E ratio between RPL and control groups, while no significance was found between RPL and control groups' NO/vitamin E ratio.

CONCLUSIONS: The decrease in NO production and vitamin E is a result of RPL and not a causative factor, as the RPL was without pathological cause, medication, or fibroid presence, and no significant difference was found between the NO/vitamin E ratio in RPL and controls group.