The use of mitomycin-C for respiratory papillomas. Clinical, histologic and biochemical correlation

OBJECTIVE: To assess the clinical effects of mitomycin-C (MMC) on human papilloma virus (HPV)-infected tissues of the airway.

METHODS: We included 10 patients with previous histologic diagnosis of recurrent respiratory papillomas (RRP) in this prospective study, conducted at the Department of Otolaryngology-Head and Neck Surgery, University of Alexandria, Egypt, between January 2000 and December 2002. Under general anesthesia, each patient underwent laser excision of all visible papillomas, followed by topical application of 1 cc of 0.5 mg/ml MMC. The procedure was repeated weekly until no visible papillomas could be microscopically detected. We histopathologically studied the obtained specimens and tested for the presence of HPV DNA using polymerase chain reaction (PCR) technique. We collected blood samples from all patients and from another 10 healthy volunteers for determination of serum interleukin-2 (IL-2) level using enzyme-linked immunosorbent assay technique.

RESULTS: We achieved clinical remission in 8 of the patients (80%), a fact that was confirmed histopathologically and by PCR data. The mean serum IL-2 levels ± SD was significantly lower in papilloma patients (83.6 ± 28.83 pg/ml) than in control subjects (196.3 ± 42.46 pg/ml) (p<0.01). Among patients with RRP, serum IL-2 levels ±SD was lower in initial samples (83.6 ± 28.83) than follow-up (95.7 ± 27.98 and 112.3 ± 33.8 and 129.4 ± 34.04) and remission samples (154 ± 37.84 pg/ml)

CONCLUSION: Our result suggests that topical application of MMC may act adjunctively to laser surgery for RRP.