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Research ArticleOriginal Article
Open Access

In vitro study assessing the response of plasmodium falciparum malaria to chloroquine, sulfadoxine/pyrimethamine, quinine and mefloquine in Wad Medani District, Sudan

Bakri Y. Nour, Ibrahim A. Faragalla, Osman K. Saeed and A. Abd Allah Mohamadani
Saudi Medical Journal June 2006, 27 (6) 808-812;
Bakri Y. Nour
Faculty of Medicine, Blue Nile Research and Training Institute, University of Gezira, PO Box 101, Wad Medani, Sudan. Tel. +249 511846183. Fax. +249 511842040. E-mail: [email protected]
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  • For correspondence: [email protected]
Ibrahim A. Faragalla
Faculty of Medicine, Blue Nile Research and Training Institute, University of Gezira, Wad Medani, Sudan.
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Osman K. Saeed
Faculty of Medicine, Blue Nile Research and Training Institute, University of Gezira, Wad Medani, Sudan.
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A. Abd Allah Mohamadani
Faculty of Medicine, Blue Nile Research and Training Institute, University of Gezira, Wad Medani, Sudan.
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Abstract

OBJECTIVE: To assess the in vitro response of Plasmodium falciparum malaria to chloroquine (CQ), sulfadoxine/pyrimethamine (SDX/PYR), Quinine (QU) and Mefloquine (MQ) and monitoring their resistance.

METHODS: In 1999 to 2000, an in vitro study was carried out in Wad Medani district in Sudan. The standard protocol of the WHO in vitro micro-test Mark II was used for the selection of the subjects, the collection of blood samples, the culture techniques, the examination of the post-culture blood slides and the interpretation of the results.

RESULTS: In vitro micro-test Mark II were performed on 62 Plasmodium falciparum isolates. Of these isolates, 42 produced successful schizonts growth. The data obtained showed that 29 of 42 isolates (69%) were CQ resistant with an effective concentrations (EC); EC50 = 399.621 nM, EC90 = 2754.145 nM and EC99 = 13284.967 nM to inhibit the schizont maturation, the values of SDX/PYR showed a flat regression line as an indication of in vitro reduced response with an EC50 = 0.262 nM, EC90 = 147.390 nM and EC99 = 25722.296 nM, and the response to the QU indicated only one of the 42 isolates (2.4%) was resistant with an EC50 = 150.085 nM, EC90 = 822.825 nM and EC99 = 3293.667 nM, while all the 42 isolates were sensitive to MQ with an EC50 = 190.763 nM, EC90 = 615.125 nM and EC99 = 1597.504 nM.

CONCLUSION: The results of this study revealed a high degree of in vitro resistance to CQ and reduced in vitro response to SDX/PYR and QU, while MQ was fully sensitive. The effective concentrations to inhibit 50, 90 and 99% of the parasite maturation were determined for antimalarial drugs efficacy monitoring surveillance in Sudan.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 27 (6)
Saudi Medical Journal
Vol. 27, Issue 6
1 Jun 2006
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In vitro study assessing the response of plasmodium falciparum malaria to chloroquine, sulfadoxine/pyrimethamine, quinine and mefloquine in Wad Medani District, Sudan
Bakri Y. Nour, Ibrahim A. Faragalla, Osman K. Saeed, A. Abd Allah Mohamadani
Saudi Medical Journal Jun 2006, 27 (6) 808-812;

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In vitro study assessing the response of plasmodium falciparum malaria to chloroquine, sulfadoxine/pyrimethamine, quinine and mefloquine in Wad Medani District, Sudan
Bakri Y. Nour, Ibrahim A. Faragalla, Osman K. Saeed, A. Abd Allah Mohamadani
Saudi Medical Journal Jun 2006, 27 (6) 808-812;
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© 2023 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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