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Research ArticleOriginal Article
Open Access

Indeterminate human immunodeficiency virus western blot results in Iranian patients with discordant screening assay results

Mehrdad Ravanshad, Fereidoun Mahboudi, Farzaneh Sabahi and Saeed Bayanolhagh
Saudi Medical Journal August 2006, 27 (8) 1130-1133;
Mehrdad Ravanshad
Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
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Fereidoun Mahboudi
Biotechnology Research Center, Pasture Institute of Iran, Tehran, Iran. Tel. +98 (21) 66480780. E-mail: [email protected]
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  • For correspondence: [email protected]
Farzaneh Sabahi
Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
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Saeed Bayanolhagh
Biotechnology Research Center, Pasture Institute of Iran, Tehran, Iran.
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Abstract

OBJECTIVE: The Western blot (WB) assay is the most widely accepted confirmatory assay for the detection and confirmation of antibodies to human immunodeficiency virus type 1 (HIV-1) and 2 (HIV-2). However, indeterminate WB reactivity to HIV-1 and HIV-2 proteins may occur in individuals who do not appear to be infected with HIV.

METHODS: In this study, we describe the results of indeterminate WB reactivity in Iranian patients with discordant screening assays. The samples were obtained from the Iranian Blood Transfusion Center, Tehran, Iran and evaluated in the Biotechnology Process Development Center, Pasteur Institute of Iran, Tehran, Iran between 2003 and 2004. A total of 4707 were tested for the presence of HIV-1 antibodies.

RESULTS: Six hundred and four (12.8%) patients tested for HIV were positive for HIV-1 antibody. Nine (1.49%) have discordant results among screening assays and indeterminate WB results as interpreted by Centers for Disease Control and Prevention (CDC) criteria. Most (66.7%) of these indeterminate WB results were due to p24 reactivity. However, 2 (22.2%) display reactivity to both gp41 and gp120 proteins [Positive by World Health Organization (WHO) criteria]. Of 9 WB assays initially indeterminate by the CDC criteria and with follow-up samples, 8 (88.8%) became negative when retested subsequently while one (11.1%) remained indeterminate for more than a year and were thus considered negative. In addition, all the indeterminate samples were negative when assessed by polymerase chain reaction assay.

CONCLUSION: In general, there was an 88.8% concordance between the CDC and WHO criteria for an indeterminate WB result. The CDC II criteria best met the specified objectives for diagnosis in our setting.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 27 (8)
Saudi Medical Journal
Vol. 27, Issue 8
1 Aug 2006
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Indeterminate human immunodeficiency virus western blot results in Iranian patients with discordant screening assay results
Mehrdad Ravanshad, Fereidoun Mahboudi, Farzaneh Sabahi, Saeed Bayanolhagh
Saudi Medical Journal Aug 2006, 27 (8) 1130-1133;

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Indeterminate human immunodeficiency virus western blot results in Iranian patients with discordant screening assay results
Mehrdad Ravanshad, Fereidoun Mahboudi, Farzaneh Sabahi, Saeed Bayanolhagh
Saudi Medical Journal Aug 2006, 27 (8) 1130-1133;
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© 2023 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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