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Research ArticleOriginal Article
Open Access

Low serum concentration facilitates the differentiation of hepatic progenitor cells

Yun He, Wei-Yu Zhang, Min Gong, Jia-Yi Huang, Ni Tang, Tao Feng, Guang-Hui Wei, Tong-Chuan He and Yang Bi
Saudi Medical Journal February 2011, 32 (2) 128-134;
Yun He
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Wei-Yu Zhang
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Min Gong
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Jia-Yi Huang
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Ni Tang
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Tao Feng
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Guang-Hui Wei
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Tong-Chuan He
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Yang Bi
Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China.
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Abstract

OBJECTIVE: To investigate the effect of serum culture conditions on the differentiation of hepatic progenitor cells (HPCs) in vitro.

METHODS: This study was conducted at the Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing, China from August 2009 to June 2010. Differentiation of HPCs was induced with dexamethasone, hepatic growth factor, and fibroblast growth factor-4 in 10% fetal bovine serum (FBS), 2% FBS, 2% horse serum (HS) conditions. First, we investigated the indirect synthesis of albumin by the albumin promoter-driven Gaussia luciferase assay, and cell proliferation by cell counting. Then, we performed reverse transcriptase-polymerase chain reaction and immunofluorescence to detect hepatic related markers Delta-like protein (DLK), cytokeratin 18 (CK18), tyrosine aminotransferase (TAT), albumin (ALB), a-fetoprotein (AFP), and glucuronosyltransferase 1A (UGT1A). Lastly, we used indocyanine green (ICG) uptake assay and periodic acid-Schiff (PAS) staining to determine the function of induced hepatocytes.

RESULTS: The proliferation of HPCs was inhibited in 2% serum culture. Induced HPCs in 2% serum exhibited higher expression of ALB, CK18, UGT1A, and TAT, whereas lower expression of DLK and AFP, compared with 10% FBS. Interestingly, 2% serum culture improved ICG uptake function, but repressed PAS staining of induced HPCs. Generally, 2% HS was better at inducing mature hepatic markers and the function of HPCs than 2% FBS.

CONCLUSION: Two percent low serum concentration conditions, especially 2% HS, improve the hepatic differentiation of HPCs, and PAS staining may not be used in induction testing of low serum concentrations.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (CC BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 32 (2)
Saudi Medical Journal
Vol. 32, Issue 2
1 Feb 2011
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Low serum concentration facilitates the differentiation of hepatic progenitor cells
Yun He, Wei-Yu Zhang, Min Gong, Jia-Yi Huang, Ni Tang, Tao Feng, Guang-Hui Wei, Tong-Chuan He, Yang Bi
Saudi Medical Journal Feb 2011, 32 (2) 128-134;

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Low serum concentration facilitates the differentiation of hepatic progenitor cells
Yun He, Wei-Yu Zhang, Min Gong, Jia-Yi Huang, Ni Tang, Tao Feng, Guang-Hui Wei, Tong-Chuan He, Yang Bi
Saudi Medical Journal Feb 2011, 32 (2) 128-134;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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