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Research ArticleOriginal Article
Open Access

Identification of Helicobacter pylori by different conventional staining techniques and its comparison with polymerase chain reaction

Himani B. Pandya, Jagdish S. Patel, Harihar H. Agravat, Sahil B. Patel and Minal C. Thakkar
Saudi Medical Journal September 2013, 34 (9) 942-948;
Himani B. Pandya
Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology, Anand 388001, Gujarat, India. Tel. +91 9898724793. Fax. +91 2692250168. E-mail: [email protected]
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Jagdish S. Patel
Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology, Anand 388001, Gujarat, India. Tel. +91 9898724793. Fax. +91 2692250168. E-mail: [email protected]
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Harihar H. Agravat
Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology, Anand 388001, Gujarat, India. Tel. +91 9898724793. Fax. +91 2692250168. E-mail: [email protected]
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Sahil B. Patel
Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology, Anand 388001, Gujarat, India. Tel. +91 9898724793. Fax. +91 2692250168. E-mail: [email protected]
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Minal C. Thakkar
Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology, Anand 388001, Gujarat, India. Tel. +91 9898724793. Fax. +91 2692250168. E-mail: [email protected]
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Abstract

OBJECTIVE: To evaluate Helicobacter pylori (H. pylori) detection by histological staining methods, and to compare with those of Gram staining and polymerase chain reaction (PCR).

METHODS: This is a cross-sectional study conducted at the Department of Microbiology, Shree P. M. Patel Paramedical College, Anand, Gujarat, India on 436 patients attending the Deep Surgical Hospital, Anand, Gujarat between February 2008 and October 2011. Biopsies were subjected to histological staining using Hematoxylin & Eosin (H&E), Giemsa, and Warthin-Starry stains, as well as with Gram staining. The PCR was performed on 71 biopsy samples.

RESULTS: Sensitivity and negative predictive values of all 3 histological stains (Warthin-Starry, H&E, and Giemsa) were excellent. Gram staining showed excellent results pertaining to sensitivity, specificity, positive predictive value, and accuracy. Sensitivity of PCR was remarkably low compared to all the staining methods. The sensitivity of all histological stains was found better than PCR.

CONCLUSION: From the findings in our study, we conclude that in a mediocre laboratory, where PCR facility is not available, histological stain can be a better substitute for the diagnosis of H. pylori. Our findings also confirm the assertion that Gram staining is a preferred stain, affordable, reliable, and simple means for identifying H. pylori compared with both histology and PCR.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (CC BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 34 (9)
Saudi Medical Journal
Vol. 34, Issue 9
1 Sep 2013
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Identification of Helicobacter pylori by different conventional staining techniques and its comparison with polymerase chain reaction
Himani B. Pandya, Jagdish S. Patel, Harihar H. Agravat, Sahil B. Patel, Minal C. Thakkar
Saudi Medical Journal Sep 2013, 34 (9) 942-948;

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Identification of Helicobacter pylori by different conventional staining techniques and its comparison with polymerase chain reaction
Himani B. Pandya, Jagdish S. Patel, Harihar H. Agravat, Sahil B. Patel, Minal C. Thakkar
Saudi Medical Journal Sep 2013, 34 (9) 942-948;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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