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Research ArticleOriginal Article
Open Access

HspX-mediated survival pathways of pathogenic mycobacteria

Fahad Alhusain
Saudi Medical Journal July 2021, 42 (7) 721-727; DOI: https://doi.org/10.15537/smj.2021.42.7.20200582
Fahad Alhusain
From the Prince Sultan Military Medical City, Riyadh, Kingdom of Saudi Arabia.
MSc
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    Figure 1

    - Growth curve for Mycobacterium bovis Bacillus Calmette-Gurin Glaxo strain.

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    Figure 2

    - Total protein concentration of Mycobacterium bovis on exposure to spermine-nitric oxide and spermine hydrochloride. Sp.NO: spermine-nitric oxide, Sp.HCl: spermine hydrochloride

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    Figure 3

    - The absorbance of standing and shaking sample concentrations of Mycobacterium bovis for the HspX protein.

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    Figure 4

    - Fluorescence intensity of Mycobacterium bovis Bacillus Calmette-Gurin Glaxo lipid bodies using 2 different incubation methods.

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    Figure 5

    - Analysis of the fluorescence intensity of Mycobacterium bovis Bacillus Calmette-Gurin Glaxo lipid bodies on exposure to nitric oxide. The production of lipid bodies in the cell culture treated with spermine-nitric oxide (black bar) increased compared with the slight increase in lipid bodies in the cell culture treated with spermine hydrochloride (gray bar).

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    Table 1

    - HspX protein concentration upon exposure to nitric oxide.

    Time course (hours)0124
    Sp.NO concentration (pg/mL)386.24598.41678.48715.61
    Sp.HCl concentration (pg/mL)294.84471.871502.85517.77
    • Sp.NO: spermine-nitric oxide, Sp.HCl: spermine hydrochloride

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Saudi Medical Journal: 42 (7)
Saudi Medical Journal
Vol. 42, Issue 7
1 Jul 2021
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HspX-mediated survival pathways of pathogenic mycobacteria
Fahad Alhusain
Saudi Medical Journal Jul 2021, 42 (7) 721-727; DOI: 10.15537/smj.2021.42.7.20200582

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HspX-mediated survival pathways of pathogenic mycobacteria
Fahad Alhusain
Saudi Medical Journal Jul 2021, 42 (7) 721-727; DOI: 10.15537/smj.2021.42.7.20200582
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Keywords

  • ELISA
  • HspX
  • hypoxia
  • lipid bodies
  • Mycobacterium bovis
  • nitric oxide

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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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