Sevoflurane reverses cisplatin resistance in neuroblastoma cells through the linc00473/miR-490-5p/AKT1 axis

- Sevoflurane reverses the cisplatin-resistant phenotype and decreases linc00473 expressions in SH-SY5Y-SR cells. A-C) SH-SY5Y-SR cells were pretreated with 5% sevoflurane and then treated with 0.1mg/l cisplatin for 24 hours. A) The morphology of SH-SY5Y-SR cells after different treatments; B) The cell viability (%) of SH-SY5Y-SR cells after different treatments were determined; C) The apoptosis rate (%) of SH-SY5Y-SR cells after different treatments were determined; D) SH-SY5Y-SR cells were treated with 5% sevoflurane and then cultured for 24 hours. RNA-seq was used to analyze the global differences in transcripts between sevoflurane treated group and control group. E-G) SH-SY5Y-SR cells were transfected with linc00473 overexpression plasmid (OV) or control plasmid (CTL) for 24 hours, and then pretreated with 5% sevoflurane and then treated with 0.1mg/l cisplatin for 24 hours. E) The relative linc00473 levels were determined by qRT-PCR assay after different treatments. F) The cell viability (%) of SH-SY5Y-SR cells after different treatments were determined; G) The apoptosis rate (%) of SH-SY5Y-SR cells after different treatments were determined.

- Linc00473 is a carcinogen of SH-SY5Y-NC cells. A) SH-SY5Y-NC cells were transfected with linc00473 overexpression plasmid (OV), siRNA (SI) or control plasmid (CTL) for 24 hours, and then the relative linc00473 levels were determined by qRT-PCR assay after different treatments. B) SH-SY5Y-NC cells were transfected with linc00473 OV or siRNA (SI) for certain period, and then the cells proliferations were determined by MTT assay. C) SH-SY5Y-NC cells were transfected with linc00473 OV, siRNA (SI) or control plasmid (CTL) for 48 hours, and then the apoptosis rates (%) and casepase-3 activity were determined.

- Linc00473 is a key factor of cisplatin resistance in SH-SY5Y-SR cells. A) Comparing the relative linc00473 levels between SH-SY5Y-SNC cells and SH-SY5Y-SR cells. B-C) SH-SY5Y-NC cells were transfected with linc00473 overexpression plasmid (OV) or control plasmid (CTL) for 24 hours, and then pretreated with 5% sevoflurane and then treated with 0.1mg/l cisplatin for 24 hours. (B) The cell viability (%) after different treatments were determined; C) The apoptosis rate (%) after different treatments were determined. D-E) SH-SY5Y-SR cells were transfected with linc00473 siRNA (SI) or control plasmid (CTL) for 24 hours, and then pretreated with 5% sevoflurane and then treated with 0.1mg/l cisplatin for 24 hours, and then D) The cell viability (%) after different treatments were determined; E) The apoptosis rate (%) after different treatments were determined.