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Research ArticleOriginal Article
Open Access

Lentivirus-mediated reversion-inducing cysteine-rich protein with Kazal motifs gene transfer suppresses pancreatic cancer invasion in vitro.

Qing-Shui Tian and De-Chun Li
Saudi Medical Journal July 2010, 31 (7) 752-758;
Qing-Shui Tian
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De-Chun Li
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Abstract

OBJECTIVES: To investigate the biological functions of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) over-expression in pancreatic cancer cell line Panc-1.

METHODS: This study was carried out in the Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China from January to September 2009. The design of the study was to construct a recombinant lentivirus carrying the RECK gene (LV-RECK) to be used to infect Panc-1 cells, and investigate the biological functions of RECK in pancreatic cancer in vitro. The RECK expression was measured by real-time polymerase chain reaction (PCR) and Western blotting. Cell proliferation and apoptosis were examined by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The ability of invasion was detected using Transwell chambers. The expression and activity of matrix metalloproteinase (MMP)-2 and matrix metalloproteinase-9 (MMP-9) was measured by real-time PCR, Western blotting, and gelatin zymography.

RESULTS: We successfully constructed LV-RECK and proved it can over-express the RECK gene in Panc-1 cells. The RECK over-expression potently suppressed the invasion ability of Panc-1 cells in vitro without affecting cell proliferation and apoptosis. The RECK over-expression potently inhibited the secretion and activity of MMP-2, and the secretion of MMP-9 without affecting the messenger ribonucleic acid, and protein expression of MMP-2 and MMP-9.

CONCLUSIONS: The RECK gene exerts repressive effects on the invasion ability of the pancreatic cancer cell line Panc-1, and it may be a novel therapeutic target for pancreatic cancer.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 31 (7)
Saudi Medical Journal
Vol. 31, Issue 7
1 Jul 2010
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Lentivirus-mediated reversion-inducing cysteine-rich protein with Kazal motifs gene transfer suppresses pancreatic cancer invasion in vitro.
Qing-Shui Tian, De-Chun Li
Saudi Medical Journal Jul 2010, 31 (7) 752-758;

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Lentivirus-mediated reversion-inducing cysteine-rich protein with Kazal motifs gene transfer suppresses pancreatic cancer invasion in vitro.
Qing-Shui Tian, De-Chun Li
Saudi Medical Journal Jul 2010, 31 (7) 752-758;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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