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Research ArticleOriginal Article
Open Access

Nucleic acid amplification technology screening for hepatitis C virus and human immunodeficiency virus for blood donations

Mohammad S. Bamaga, Fawzi F. Bokhari, AbdulRahaman M. Aboud, Meshal Al-Malki and Faris Q. Alenzi
Saudi Medical Journal June 2006, 27 (6) 781-787;
Mohammad S. Bamaga
Department of Molecular Pathology, Al-Hada Armed Forces Hospital, PO Box 1347, HHRC 479, Taif, Kingdom of Saudi Arabia. Tel. +966 (2) 7541610/1028. Fax. +966 (2) 7541238. E-mail: [email protected]
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Fawzi F. Bokhari
Department of Molecular Pathology, Al-Hada Armed Forces Hospital, Taif, Kingdom of Saudi Arabia.
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AbdulRahaman M. Aboud
Department of Molecular Pathology, Al-Hada Armed Forces Hospital, Taif, Kingdom of Saudi Arabia.
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Meshal Al-Malki
Department of Molecular Pathology, Al-Hada Armed Forces Hospital, Taif, Kingdom of Saudi Arabia.
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Faris Q. Alenzi
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, Dammam, Kingdom of Saudi Arabia.
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Abstract

OBJECTIVE: To investigate the performance of the commercial Roche COBAS AmpliScreen assay, and demonstrate whether the COBAS AmpliScreen human immunodeficiency virus-1 (HIV-1) test, v1.5, and COBAS AmpliScreen hepatitis C virus (HCV) v 2.0 for screening for HIV-1 and HCV RNA in the donated blood units from which plasma mini pools were collected, by nucleic acid amplification technology (NAT), could detect the positive pools and reduce the risk of transmission of infections for those routinely tested by serological assays.

METHODS: The study was performed on 3288 plasma samples collected from blood donors in a period of 13 months, from August 2004 to August 2005, at Al-Hada Armed Forces Hospital, Molecular Pathology Laboratory, Taif, Kingdom of Saudi Arabia. The samples were tested by the reverse transcriptase polymerase chain reaction (RT-PCR) after RNA extraction (this represents the major method in NAT assays), in parallel with the routine serological testing to detect qualitatively for HIV-1 and HCV.

RESULTS: The NAT assays that include an automated COBAS AmpliPrep system for RNA extraction and COBAS Amplicor Analyzer using AmpliScreen kits for RT-PCR assays, and the routine serological screening assays for the detection of the HIV-1 and HCV RNA in the plasma samples from the blood donors have shown to be a reliable combination that would meet our requirements. The collected data further confirms the results from the serological assays and enables us to decrease the residual risk of transmission to a minimum with the finding of no seronegative window period donation. The results demonstrate that out of 3288 samples, the percentages of RT-PCR (NAT) negative blood donations that were also confirmed as seronegative were 99% for HCV, and 99.1% for HIV-1.

CONCLUSION: The modified combined systems (automated COBAS AmpliPrep system for RNA extraction and COBAS Amplicor Analyzer using AmpliScreen kits for RT-PCR assays) for NAT screening assays has allowed the release of all blood donations supplied in the specified period of the study with no seronegative window period donations. This facilitates keeping the residual risk of transmission of HIV-1 and HCV to its minimum through blood transfusion.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 27 (6)
Saudi Medical Journal
Vol. 27, Issue 6
1 Jun 2006
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Nucleic acid amplification technology screening for hepatitis C virus and human immunodeficiency virus for blood donations
Mohammad S. Bamaga, Fawzi F. Bokhari, AbdulRahaman M. Aboud, Meshal Al-Malki, Faris Q. Alenzi
Saudi Medical Journal Jun 2006, 27 (6) 781-787;

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Nucleic acid amplification technology screening for hepatitis C virus and human immunodeficiency virus for blood donations
Mohammad S. Bamaga, Fawzi F. Bokhari, AbdulRahaman M. Aboud, Meshal Al-Malki, Faris Q. Alenzi
Saudi Medical Journal Jun 2006, 27 (6) 781-787;
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© 2023 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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