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Research ArticleOriginal Article
Open Access

Comparison of immunoassays for differentiation of herpes simplex virus type 1 and 2 antibodies

Mohammed O. Qutub, Paul E. Klapper, Pam J. Vallely, Gerham M. Cleator and Daniel Mandall
Saudi Medical Journal June 2006, 27 (6) 788-793;
Mohammed O. Qutub
Division of Clinical Microbiology, MBC#10, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Kingdom of Saudi Arabia. Fax. +966 (1) 4424280. E-mail: [email protected]
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  • For correspondence: [email protected]
Paul E. Klapper
Division of Virology, Department of Pathological Sciences, University of Manchester, Manchester, United Kingdom.
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Pam J. Vallely
Division of Virology, Department of Pathological Sciences, University of Manchester, Manchester, United Kingdom.
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Gerham M. Cleator
Division of Virology, Department of Pathological Sciences, University of Manchester, Manchester, United Kingdom.
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Daniel Mandall
Manchester Centre for Sexual Health, Central Manchester Healthcare Trust, Manchester, United Kingdom
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Abstract

OBJECTIVE: To asses the commercial available enzyme-linked immunosorbent assays (ELISA) for differentiation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) antibodies.

METHODS: The study was performed between January 1997 to November 2002 in the Division of Virology, Department of Pathological Sciences, Central Manchester Healthcare Trust and University of Manchester, Manchester, United Kingdom. Assays based upon type-specific glycoprotein G-1 (gG-1) for HSV-1, and glycoprotein G-2 (gG-2) from HSV-2 were evaluated to differentiate between HSV-1 and HSV-2 antibodies. Using 5 different ELISA tests, 2 panels of serum samples were tested. Panel one consisted of 88 sera, selected from the serum bank of the Clinical Virology Laboratory, Manchester Royal Infirmary; panel 2 comprised of 90 sera selected from samples collected from Bangladeshi female commercial workers.

RESULTS: The data of this study showed that a high rate of gG-1 based immunoassays ranged from 87.9-100% for sensitivity and 51.5-100% specificity.

CONCLUSION: Although there are several immunoassays were claimed to differentiate between HSV-1 and HSV-2 antibodies, selection of these assays should be carefully interpreted with the overall clinical framework provided by detailed sexual history and genital examination.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 27 (6)
Saudi Medical Journal
Vol. 27, Issue 6
1 Jun 2006
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Comparison of immunoassays for differentiation of herpes simplex virus type 1 and 2 antibodies
Mohammed O. Qutub, Paul E. Klapper, Pam J. Vallely, Gerham M. Cleator, Daniel Mandall
Saudi Medical Journal Jun 2006, 27 (6) 788-793;

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Comparison of immunoassays for differentiation of herpes simplex virus type 1 and 2 antibodies
Mohammed O. Qutub, Paul E. Klapper, Pam J. Vallely, Gerham M. Cleator, Daniel Mandall
Saudi Medical Journal Jun 2006, 27 (6) 788-793;
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© 2023 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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