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Research ArticleOriginal Article
Open Access

Polo-like kinase-1 regulates first cleavage of one-cell embryos in culture during assisted reproduction

Yiqing Zhao, Jihui Ai, Hanwang Zhang and Guijin Zhu
Saudi Medical Journal March 2010, 31 (3) 247-252;
Yiqing Zhao
Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
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Jihui Ai
Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
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Hanwang Zhang
Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
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Guijin Zhu
Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
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Abstract

OBJECTIVE: To investigate the role of polo-like kinase -1 (PLK1) in the first cleavage of a zygote in culture.

METHODS: This experiment took place in the Reproductive Medicine Center of Tongji Hospital, Wuhan, China, from 1st June 2009 to 20th November 2009. First, we detected the expression of PLK1 during the first zygotic division by using Western blotting, and then we reduced the expression of PLK1 during the first zygotic division by ribonucleic acid (RNA) interference (including 4 groups: PLK1 small interfering RNA (siRNA), siRNA control, mock transfection, and only zona pellucida (ZP) removal), finally we evaluated and compared the first cleavage rates of the 4 groups.

RESULTS: The expression of PLK1 peaked in the first M phase of zygotic cleavage (3 samples/group, 100 zygotes/sample). The relative amount of PLK1 of the mouse zygotes was reduced significantly after siRNA transfection. The first cleavage rate of the PLK1 siRNA group was significantly less than that of other groups (siRNA control, mock transfection, and only ZP removal, p=0.000).

CONCLUSION: The PLK1 plays a crucial role during the first cleavage of one-cell embryos, and the zygotes are unable to divide successfully without functional PLK1.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (CC BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 31 (3)
Saudi Medical Journal
Vol. 31, Issue 3
1 Mar 2010
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Polo-like kinase-1 regulates first cleavage of one-cell embryos in culture during assisted reproduction
Yiqing Zhao, Jihui Ai, Hanwang Zhang, Guijin Zhu
Saudi Medical Journal Mar 2010, 31 (3) 247-252;

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Polo-like kinase-1 regulates first cleavage of one-cell embryos in culture during assisted reproduction
Yiqing Zhao, Jihui Ai, Hanwang Zhang, Guijin Zhu
Saudi Medical Journal Mar 2010, 31 (3) 247-252;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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