Study on related indexes of the coagulation and fibrinolytic system after renal ischemia reperfusion injury in Wistar rats

OBJECTIVE: To explore changes in indicators of the coagulation and fibrinolytic system in the serum of rats with renal ischemia reperfusion (IR) injury.

METHODS: The study was carried out at the Nephrology Laboratory of the Second Hospital, Shanxi Medical University, Taiyuan, China between September and October 2012. A rat renal IR model was established, and serum samples of each group (8 each) were collected to detect the amounts of fibrinogen (FIB), antithrombin III (AT-III), tissue type plasminogen activator (tPA), plasminogen-antiplasmin complex (PAP), and plasminogen activator inhibitor-1 (PAI-1).

RESULTS: After the start of reperfusion, the changes of FIB exhibited a ‘double-peak’ appearance. The AT-III began to decline at 2 hours to the lowest level at 12 hours, and then gradually recovered. The tPA declined to the lowest level at 12 hours, and then increased to the highest level at 24 hours. The PAI-1 began to increase at 6 hours, and peaked at 24 hours, and then recovered gradually. The PAP peaked at 6 hours, and declined to the lowest level at 12 hours, and began to recover at 24 hours, and was normal at 36 hours. All the above indexes were completely restored at 48 hours.

CONCLUSION: At 2 hours after reperfusion, the coagulation system were activated, reaching a peak at 12 hours. Fibrinolytic and antifibrinolytic substances were activated at 2 hours, and the fibrinolytic system was inhibited at 6 hours, reaching a peak at 24 hours. Coagulation and fibrinolytic substances were restored to normal at 48 hours.