Skip to main content

Main menu

  • Home
  • Content
    • Latest
    • Archive
    • home
  • Info for
    • Authors
    • Reviewers
    • Subscribers
    • Institutions
    • Advertisers
    • Join SMJ
  • About Us
    • About Us
    • Editorial Office
    • Editorial Board
  • More
    • Advertising
    • Alerts
    • Feedback
    • Folders
    • Help
  • Other Publications
    • NeuroSciences Journal

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Saudi Medical Journal
  • Other Publications
    • NeuroSciences Journal
  • My alerts
  • Log in
Saudi Medical Journal

Advanced Search

  • Home
  • Content
    • Latest
    • Archive
    • home
  • Info for
    • Authors
    • Reviewers
    • Subscribers
    • Institutions
    • Advertisers
    • Join SMJ
  • About Us
    • About Us
    • Editorial Office
    • Editorial Board
  • More
    • Advertising
    • Alerts
    • Feedback
    • Folders
    • Help
  • Follow psmmc on Twitter
  • Visit psmmc on Facebook
  • RSS
Research ArticleOriginal Article
Open Access

Molecular characterization of Helicobacter pylori clinical isolates from Eastern Saudi Arabia

Khaled R. Alkharsah, Reem Y. Aljindan, Aisha M. Alamri, Amer I. Alomar and Abdulaziz A. Al-Quorain
Saudi Medical Journal October 2022, 43 (10) 1128-1135; DOI: https://doi.org/10.15537/smj.2022.43.10.20220355
Khaled R. Alkharsah
From the Department of Microbiology (Alkharsah, Aljindan), College of Medicine; from the Department of Clinical Laboratory Sciences (Alamri, Alomar), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, and from the Department of Gastroenterology (Al-Quorain), King Fahd Hospital of the University, Alkhobar, Kingdom of Saudi Arabia.
PhD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: [email protected]
Reem Y. Aljindan
From the Department of Microbiology (Alkharsah, Aljindan), College of Medicine; from the Department of Clinical Laboratory Sciences (Alamri, Alomar), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, and from the Department of Gastroenterology (Al-Quorain), King Fahd Hospital of the University, Alkhobar, Kingdom of Saudi Arabia.
PhD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aisha M. Alamri
From the Department of Microbiology (Alkharsah, Aljindan), College of Medicine; from the Department of Clinical Laboratory Sciences (Alamri, Alomar), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, and from the Department of Gastroenterology (Al-Quorain), King Fahd Hospital of the University, Alkhobar, Kingdom of Saudi Arabia.
PhD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amer I. Alomar
From the Department of Microbiology (Alkharsah, Aljindan), College of Medicine; from the Department of Clinical Laboratory Sciences (Alamri, Alomar), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, and from the Department of Gastroenterology (Al-Quorain), King Fahd Hospital of the University, Alkhobar, Kingdom of Saudi Arabia.
PhD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Abdulaziz A. Al-Quorain
From the Department of Microbiology (Alkharsah, Aljindan), College of Medicine; from the Department of Clinical Laboratory Sciences (Alamri, Alomar), College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, and from the Department of Gastroenterology (Al-Quorain), King Fahd Hospital of the University, Alkhobar, Kingdom of Saudi Arabia.
MD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • eLetters
  • Info & Metrics
  • References
  • PDF
Loading

References

  1. 1.↵
    1. Piscione M,
    2. Mazzone M,
    3. Di Marcantonio MC,
    4. Muraro R,
    5. Mincione G.
    Eradication of Helicobacter pylori and gastric cancer: a controversial relationship. Front Microbiol 2021; 12: 630852.
    OpenUrl
  2. 2.↵
    1. Ghotaslou R,
    2. Leylabadlo HE,
    3. Asl YM.
    Prevalence of antibiotic resistance in Helicobacter pylori: a recent literature review. World J Methodol 2015; 5: 164-174.
    OpenUrlCrossRefPubMed
  3. 3.↵
    1. Savoldi A,
    2. Carrara E,
    3. Graham DY,
    4. Conti M,
    5. Tacconelli E.
    Prevalence of antibiotic resistance in Helicobacter pylori: a systematic review and meta-analysis in World Health Organization regions. Gastroenterology 2018; 155: 1372-1382.
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Liu Y,
    2. Wang S,
    3. Yang F,
    4. Chi W,
    5. Ding L,
    6. Liu T, et al.
    Antimicrobial resistance patterns and genetic elements associated with the antibiotic resistance of Helicobacter pylori strains from Shanghai. Gut Pathog 2022; 14: 14.
    OpenUrl
  5. 5.↵
    1. Hirata K,
    2. Suzuki H,
    3. Nishizawa T,
    4. Tsugawa H,
    5. Muraoka H,
    6. Saito Y, et al.
    Contribution of efflux pumps to clarithromycin resistance in Helicobacter pylori. J Gastroenterol Hepatol 2010; 25: S75-S79.
    OpenUrlCrossRefPubMed
  6. 6.↵
    1. Binh TT,
    2. Shiota S,
    3. Suzuki R,
    4. Matsuda M,
    5. Trang TT,
    6. Kwon DH, et al.
    Discovery of novel mutations for clarithromycin resistance in Helicobacter pylori by using next-generation sequencing. J Antimicrob Chemother 2014; 69: 1796-1803.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Bińkowska A,
    2. Biernat MM,
    3. Łaczmański Ł,
    4. Gościniak G.
    Molecular patterns of resistance among Helicobacter pylori strains in South-Western Poland. Front Microbiol 2018; 9: 3154.
    OpenUrl
  8. 8.↵
    1. Seck A,
    2. Burucoa C,
    3. Dia D,
    4. Mbengue M,
    5. Onambele M,
    6. Raymond J, et al.
    Primary antibiotic resistance and associated mechanisms in Helicobacter pylori isolates from Senegalese patients. Ann Clin Microbiol Antimicrob 2013; 12: 3.
    OpenUrl
  9. 9.↵
    1. Hanafi A,
    2. Lee WC,
    3. Loke MF,
    4. Teh X,
    5. Shaari A,
    6. Dinarvand M, et al.
    Molecular and proteomic analysis of levofloxacin and metronidazole resistant Helicobacter pylori. Front Microbiol 2016; 7: 2015.
    OpenUrlCrossRef
  10. 10.↵
    1. Megraud F,
    2. Coenen S,
    3. Versporten A,
    4. Kist M,
    5. Lopez-Brea M,
    6. Hirschl AM, et al.
    Helicobacter pylori resistance to antibiotics in Europe and its relationship to antibiotic consumption. Gut 2013; 62: 34-42.
    OpenUrlAbstract/FREE Full Text
  11. 11.↵
    1. Akeel M,
    2. Shehata A,
    3. Elhafey A,
    4. Elmakki E,
    5. Aboshouk T,
    6. Ageely H, et al.
    Helicobacter pylori vacA, cagA and iceA genotypes in dyspeptic patients from southwestern region, Saudi Arabia: distribution and association with clinical outcomes and histopathological changes. BMC Gastroenterol 2019; 19: 16.
    OpenUrl
  12. 12.↵
    1. Bridge DR,
    2. Merrell DS.
    Polymorphism in the Helicobacter pylori CagA and VacA toxins and disease. Gut Microbes 2013; 4: 101-117.
    OpenUrlCrossRef
  13. 13.↵
    1. Alghamdi TS,
    2. Ansari T,
    3. Bashir AA,
    4. Batais MA,
    5. Aldhahi MF,
    6. Alanazi MA.
    Helicobacter Pylori infection among dyspepsia patients in suburbs of Riyadh, Saudi Arabia. J Pak Med Assoc 2020; 70: 2174-2177.
    OpenUrl
  14. 14.
    1. Momenah AM,
    2. Tayeb MT.
    Helicobacter pylori cagA and iceA genotypes status and risk of peptic ulcer in Saudi patients. Saudi Med J 2007; 28: 382-385.
    OpenUrlAbstract/FREE Full Text
  15. 15.
    1. Elsawaf ZM,
    2. Albasri AM,
    3. Hussainy AS,
    4. Alhujaily AS.
    Histopathological pattern of benign endoscopic gastric biopsies in Western Saudi Arabia: a review of 1236 cases. J Pak Med Assoc 2017; 67: 252-255.
    OpenUrl
  16. 16.↵
    1. Akeel M,
    2. Elmakki E,
    3. Shehata A,
    4. Elhafey A,
    5. Aboshouk T,
    6. Ageely H, et al.
    Prevalence and factors associated with H. pylori infection in Saudi patients with dyspepsia. Electron Physician 2018; 10: 7279-7286.
    OpenUrl
  17. 17.↵
    1. Al-Hussaini AA,
    2. Al Jurayyan AN,
    3. Bashir SM,
    4. Alshahrani D.
    Where are we today with Helicobacter pylori infection among healthy children in Saudi Arabia? Saudi J Gastroenterol 2019; 25: 309-318.
    OpenUrl
  18. 18.↵
    1. Falsafi T,
    2. Favaedi R,
    3. Mahjoub F,
    4. Najafi M.
    Application of stool-PCR test for diagnosis of Helicobacter pylori infection in children. World J Gastroenterol 2009; 15: 484-488.
    OpenUrlCrossRefPubMedWeb of Science
  19. 19.↵
    1. Park CY,
    2. Kwak M,
    3. Gutierrez O,
    4. Graham DY,
    5. Yamaoka Y.
    Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures. J Clin Microbiol 2003; 41: 3336-3338.
    OpenUrlAbstract/FREE Full Text
  20. 20.↵
    1. Chen J,
    2. Ye L,
    3. Jin L,
    4. Xu X,
    5. Xu P,
    6. Wang X, et al.
    Application of next-generation sequencing to characterize novel mutations in clarithromycin-susceptible Helicobacter pylori strains with A2143G of 23s rRNA gene. Ann Clin Microbiol Antimicrob 2018; 17: 10.
    OpenUrlCrossRef
  21. 21.↵
    1. Saber T,
    2. Ghonaim MM,
    3. Yousef AR,
    4. Khalifa A,
    5. Al Qurashi H,
    6. Shaqhan M, et al.
    Association of Helicobacter pylori cagA gene with gastric cancer and peptic ulcer in Saudi patients. J Microbiol Biotechnol 2015; 25: 1146-1153.
    OpenUrl
  22. 22.↵
    1. Marie MA.
    Relationship between Helicobacter pylori virulence genes and clinical outcomes in Saudi patients. J Korean Med Sci 2012; 27: 190-193.
    OpenUrlPubMed
  23. 23.↵
    1. Backert S,
    2. Tegtmeyer N,
    3. Fischer W. Composition
    , structure and function of the Helicobacter pylori cag pathogenicity island encoded type IV secretion system. Future Microbiol 2015; 10: 955-965.
    OpenUrlCrossRef
  24. 24.↵
    1. Kontizas E,
    2. Tastsoglou S,
    3. Karamitros T,
    4. Karayiannis Y,
    5. Kollia P,
    6. Hatzigeorgiou AG, et al.
    Impact of Helicobacter pylori infection and its major virulence factor CagA on DNA damage repair. Microorganisms 2020; 8: 2007.
    OpenUrl
  25. 25.↵
    1. Horiuchi S,
    2. Nakano R,
    3. Nakano A,
    4. Hishiya N,
    5. Uno K,
    6. Suzuki Y, et al.
    Prevalence of Helicobacter pylori among residents and their environments in the Nara prefecture, Japan. J Infect Public Health 2021; 14: 271-275.
    OpenUrl
  26. 26.↵
    1. Palframan SL,
    2. Kwok T,
    3. Gabriel K.
    Vacuolating cytotoxin A (VacA), a key toxin for Helicobacter pylori pathogenesis. Front Cell Infect Microbiol 2012; 2: 92.
    OpenUrl
  27. 27.↵
    1. Figueroa G,
    2. Troncoso M,
    3. Toledo MS,
    4. Faúndez G,
    5. Acuña R.
    Prevalence of serum antibodies to Helicobacter pylori VacA and CagA and gastric diseases in Chile. J Med Microbiol 2002; 51: 300-304.
    OpenUrlPubMed
  28. 28.↵
    1. Monroy FP,
    2. Brown HE,
    3. Sanderson PR,
    4. Jarrin G,
    5. Mbegbu M,
    6. Kyman S, et al.
    Helicobacter pylori in Native Americans in Northern Arizona. Diseases 2022; 10: 19.
    OpenUrl
  29. 29.↵
    1. McClain MS,
    2. Beckett AC,
    3. Cover TL.
    Helicobacter pylori vacuolating toxin and gastric cancer. Toxins (Basel) 2017; 9: 316.
    OpenUrl
  30. 30.↵
    1. Yamaoka Y,
    2. Kodama T,
    3. Kita M,
    4. Imanishi J,
    5. Kashima K,
    6. Graham DY.
    Relationship of vacA genotypes of Helicobacter pylori to cagA status, cytotoxin production, and clinical outcome. Helicobacter 1998; 3: 241-253.
    OpenUrlCrossRefPubMedWeb of Science
  31. 31.↵
    1. Al-Khattaf AS.
    Helicobacter pylori virulence markers in gastroduodenal disorders. Detection of cytotoxin-associated gene A and vacuolating cytotoxin-associated gene A genes in Saudi patients. Saudi Med J 2012; 33: 716-721.
    OpenUrlPubMed
  32. 32.↵
    1. Momenah AM,
    2. Tayeb MT.
    Relationship between Helicobacter pylori vacA genotypes status and risk of peptic ulcer in Saudi patients. Saudi Med J 2006; 27: 804-807.
    OpenUrlAbstract/FREE Full Text
  33. 33.↵
    1. Bibi F,
    2. Alvi SA,
    3. Sawan SA,
    4. Yasir M,
    5. Sawan A,
    6. Jiman-Fatani AA, et al.
    Detection and genotyping of Helicobacter pylori among gastric ulcer and cancer patients from Saudi Arabia. Pak J Med Sci 2017; 33: 320-324.
    OpenUrl
  34. 34.↵
    1. Kamboj AK,
    2. Cotter TG,
    3. Oxentenko AS.
    Helicobacter pylori: the past, present, and future in management. Mayo Clin Proc 2017; 92: 599-604.
    OpenUrlCrossRef
  35. 35.↵
    1. Eed EM,
    2. Hawash YA,
    3. Khalifa AS,
    4. Alsharif KF,
    5. Alghamdi SA,
    6. Saber T, et al.
    Molecular diagnosis of Helicobacter pylori antibiotic resistance in the Taif region, Saudi Arabia. Microbiol Immunol 2019; 63: 199-205.
    OpenUrl
  36. 36.↵
    1. Phan TN,
    2. Santona A,
    3. Tran VH,
    4. Tran TN,
    5. Le VA,
    6. Cappuccinelli P, et al.
    High rate of levofloxacin resistance in a background of clarithromycin- and metronidazole-resistant Helicobacter pylori in Vietnam. Int J Antimicrob Agents 2015; 45: 244-248.
    OpenUrl
  37. 37.↵
    1. Waskito LA,
    2. Salama NR,
    3. Yamaoka Y.
    Pathogenesis of Helicobacter pylori infection. Helicobacter 2018; 23: e12516.
    OpenUrl
  38. 38.↵
    1. Domanovich-Asor T,
    2. Motro Y,
    3. Khalfin B,
    4. Craddock HA,
    5. Peretz A,
    6. Moran-Gilad J.
    Genomic analysis of antimicrobial resistance genotype-to-phenotype agreement in Helicobacter pylori. Microorganisms 2020; 9: 2.
    OpenUrl
  39. 39.↵
    1. Hussein RA,
    2. Al-Ouqaili MTS,
    3. Majeed YH.
    Detection of clarithromycin resistance and 23srRNA point mutations in clinical isolates of Helicobacter pylori isolates: phenotypic and molecular methods. Saudi J Biol Sci 2022; 29: 513-520.
    OpenUrl
  40. 40.↵
    1. Zhang Y,
    2. Wen Y,
    3. Xiao Q,
    4. Zheng W,
    5. Long G,
    6. Chen B, et al.
    Mutations in the antibiotic target genes related to clarithromycin, metronidazole and levofloxacin resistance in Helicobacter pylori strains from children in China. Infect Drug Resist 2020; 13: 311-322.
    OpenUrl
PreviousNext
Back to top

In this issue

Saudi Medical Journal: 43 (10)
Saudi Medical Journal
Vol. 43, Issue 10
1 Oct 2022
  • Table of Contents
  • Cover (PDF)
  • Index by author
Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Saudi Medical Journal.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Molecular characterization of Helicobacter pylori clinical isolates from Eastern Saudi Arabia
(Your Name) has sent you a message from Saudi Medical Journal
(Your Name) thought you would like to see the Saudi Medical Journal web site.
Citation Tools
Molecular characterization of Helicobacter pylori clinical isolates from Eastern Saudi Arabia
Khaled R. Alkharsah, Reem Y. Aljindan, Aisha M. Alamri, Amer I. Alomar, Abdulaziz A. Al-Quorain
Saudi Medical Journal Oct 2022, 43 (10) 1128-1135; DOI: 10.15537/smj.2022.43.10.20220355

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Molecular characterization of Helicobacter pylori clinical isolates from Eastern Saudi Arabia
Khaled R. Alkharsah, Reem Y. Aljindan, Aisha M. Alamri, Amer I. Alomar, Abdulaziz A. Al-Quorain
Saudi Medical Journal Oct 2022, 43 (10) 1128-1135; DOI: 10.15537/smj.2022.43.10.20220355
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
    • Abstract
    • Methods
    • Results
    • Discussion
    • Acknowledgment
    • Footnotes
    • References
  • Figures & Data
  • eLetters
  • References
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • The risk factors for cardiovascular disease and chronic kidney disease in patients with nonalcoholic fatty liver disease in Saudi Arabia
  • Prolonged flight exposure and its effects on sinonasal health among aircrew members
  • Identifying individuals at risk of post-stroke depression
Show more Original Article

Similar Articles

Keywords

  • H. Pylori
  • clarithromycin
  • resistance
  • Saudi Arabia
  • 23s rRNA

CONTENT

  • home

JOURNAL

  • home

AUTHORS

  • home
Saudi Medical Journal

© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

Powered by HighWire