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Research ArticleOriginal Article
Open Access

Leptin expression by heterotopic ossification-isolated tissue in rats with Achilles' tenotomy

Jun-Chang Xu, Tao Wu, Gui-Hua Wu, Zhao-Ming Zhong, Yong-Zhi Tang and Jian-Ting Chen
Saudi Medical Journal May 2009, 30 (5) 605-610;
Jun-Chang Xu
Department of Spine & Orthopedics,Nanfang Hospital of Southern Medical University, Guangdong Province, China.
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Tao Wu
Department of Spine & Orthopedics,Nanfang Hospital of Southern Medical University, Guangdong Province, China.
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Gui-Hua Wu
Department of Spine & Orthopedics,Nanfang Hospital of Southern Medical University, Guangdong Province, China.
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Zhao-Ming Zhong
Department of Spine & Orthopedics,Nanfang Hospital of Southern Medical University, Guangdong Province, China.
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Yong-Zhi Tang
Department of Spine & Orthopedics,Nanfang Hospital of Southern Medical University, Guangdong Province, China.
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Jian-Ting Chen
Department of Spine & Orthopedics,Nanfang Hospital of Southern Medical University, Guangdong Province, China.
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Abstract

OBJECTIVE: To set up heterotopic ossification (HO) models according to McClure and determine whether leptin messenger ribonucleic acid (mRNA) and protein are expressed in HO-isolated tissue.

METHODS: This study was performed in the Department of Spine and Orthopedics, Southern Medical University, Guangzhou, China from November 2007 to June 2008. Thirty-six male rats were randomly divided into sham, partial achilles' tenotomy (PAT), and achilles' tenotomy (AT) groups, with 12 rats in each group. X-ray and histological examination were carried out to ensure the formation of HO at 5 and 10 weeks after operation. Specimens from achilles tendons and surrounding tissue were taken and processed. Meanwhile, the expression of leptin mRNA (5 and 10 weeks) and protein (10 weeks) in achilles tendons and the surrounding tissue were examined respectively using reverse transcription-polymerase chain reaction assay and immunohistochemical methods.

RESULTS: There were no leptin mRNA and protein expression in the sham and a weak expression in the PAT of Achilles tendons and surrounding tissue, whereas there was strong expression in the AT group.

CONCLUSION: Leptin is involved in the formation of HO, its mechanisms is related to induction of bone formation and maturation through a series of cellular events, including: proliferation/differentiation of many kinds of cells, collagen synthesis, mineralization, and vascularization of the extracellular matrix.

  • Copyright: © Saudi Medical Journal

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saudi Medical Journal: 30 (5)
Saudi Medical Journal
Vol. 30, Issue 5
1 May 2009
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Leptin expression by heterotopic ossification-isolated tissue in rats with Achilles' tenotomy
Jun-Chang Xu, Tao Wu, Gui-Hua Wu, Zhao-Ming Zhong, Yong-Zhi Tang, Jian-Ting Chen
Saudi Medical Journal May 2009, 30 (5) 605-610;

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Leptin expression by heterotopic ossification-isolated tissue in rats with Achilles' tenotomy
Jun-Chang Xu, Tao Wu, Gui-Hua Wu, Zhao-Ming Zhong, Yong-Zhi Tang, Jian-Ting Chen
Saudi Medical Journal May 2009, 30 (5) 605-610;
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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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