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Research ArticleOriginal Article
Open Access

Legionella confirmation in cooling tower water

Comparison of culture, real-time PCR and next generation sequencing

Maha Farhat, Raja A. Shaheed, Haidar H. Al-Ali, Abdullah S. Al-Ghamdi, Ghadeer M. Al-Hamaqi, Hawraa S. Maan, Zainab A. Al-Mahfoodh and Hussain Z. Al-Seba
Saudi Medical Journal February 2018, 39 (2) 137-141; DOI: https://doi.org/10.15537/smj.2018.2.21587
Maha Farhat
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MSc, PhD
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  • For correspondence: [email protected]
Raja A. Shaheed
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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Haidar H. Al-Ali
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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Abdullah S. Al-Ghamdi
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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Ghadeer M. Al-Hamaqi
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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Hawraa S. Maan
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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Zainab A. Al-Mahfoodh
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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Hussain Z. Al-Seba
From the Department of Biochemistry (Farhat), Department of Epidemic Diseases Research (Farhat), College of Medicine (Shaheed, Aall-Ali, Al-Ghamdi, Al-Hamaqi, Maan, Al-Mahfoodh, Al-Seba), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia
MBBS
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    Figure 1

    Legionella concentrations (mean ± 2SD) in cooling tower water by real-time PCR.

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    Figure 2

    Bacterial community composition of water libraries W1 and W2.

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    Figure 3

    Bacterial phyla detected in water libraries W1 and W2.

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    Figure 4

    Bacterial genera detected in water libraries W1 and W2.

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Saudi Medical Journal: 39 (2)
Saudi Medical Journal
Vol. 39, Issue 2
1 Feb 2018
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Legionella confirmation in cooling tower water
Maha Farhat, Raja A. Shaheed, Haidar H. Al-Ali, Abdullah S. Al-Ghamdi, Ghadeer M. Al-Hamaqi, Hawraa S. Maan, Zainab A. Al-Mahfoodh, Hussain Z. Al-Seba
Saudi Medical Journal Feb 2018, 39 (2) 137-141; DOI: 10.15537/smj.2018.2.21587

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Legionella confirmation in cooling tower water
Maha Farhat, Raja A. Shaheed, Haidar H. Al-Ali, Abdullah S. Al-Ghamdi, Ghadeer M. Al-Hamaqi, Hawraa S. Maan, Zainab A. Al-Mahfoodh, Hussain Z. Al-Seba
Saudi Medical Journal Feb 2018, 39 (2) 137-141; DOI: 10.15537/smj.2018.2.21587
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