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Research ArticleOriginal Article
Open Access

Does the preoperative fasting period affect the liver in a distant organ model of renal ischaemia reperfusion?

Yasemin Akcaalan, Ersin Gurkan Dumlu, Ebru Menekse, Mustafa Cem Yilmaz, Ezgi Erkilic, Betul Ogut and Aylin Sepıcı Dıncel
Saudi Medical Journal February 2023, 44 (2) 171-177; DOI: https://doi.org/10.15537/smj.2023.44.2.20220582
Yasemin Akcaalan
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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  • For correspondence: [email protected]
Ersin Gurkan Dumlu
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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Ebru Menekse
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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Mustafa Cem Yilmaz
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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Ezgi Erkilic
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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Betul Ogut
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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Aylin Sepıcı Dıncel
From the Department of Anesthesia and Reanimation (Akcaalan, Yilmaz, Erkilic), Ankara City Hospital, from the Department of General Surgery (Dumlu, Menekse), Ankara Yıldırım Beyazıt University, from the Department of Pathology (Ogut); and from the Department of Biochemistry (Dıncel), Medicine Faculty, Ankara Gazi University, Ankara, Turkey.
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    Figure 1

    - Variation of cytokine levels in pre-op and post-op blood of rats. A) Statistically significant difference was determined between the groups in respect of postoperative IL-10 levels (p=0.020) with significantly lower levels determined in Group C than in Groups A and B (p=0.021). B) Within the groups, there was no statistically significant difference between the preoperative and postoperative IL-6 levels according to Bonferroni correction (p>0.167). C) Within the groups, there was no statistically significant difference between the preoperative and postoperative TNF-α levels according to Bonferroni correction (p>0.167). D) According to Bonferroni correction, there was no statistically significant difference between the groups in respect of preoperative IL-1β levels (p=0.028). IL-6: interleukin 6, IL-10: interleukin 10, TNF-α: tumor necrosis factor alpha, Pre-op: preoperative, Post-op: postoperative, IL-1β: interleukin-1 beta

  • Figure 2
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    Figure 2

    - Histopathological images of liver tissue in rats. a) Normal liver parenchyma (Group C, Rat 1), showing no congestion, nuclear pleomorphism, or ballooning degeneration at high power (200X). b) No parenchymal or portal neutrophil leukocyte infiltration was observed in any liver tissue at high power (40X). c) Liver tissue with congestion score 0 (Group C, Rat 4); and d) at high power (40X). e & f) Congestion score 2 (Group B, Rat 4) at high power (200X). Biopsies with ballooning degeneration and nuclear pleomorphism.

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    Table 1

    - The scores related to the histopathological markers in the liver tissues of the groups.

    Histopathological markersGroup AGroup BGroup C
    Congestion
    0--4 (57.1)
    14 (57.1)3 (42.9)1 (14.3)
    23 (42.9)4 (57.1)2 (28.6)
    Vacuolar degeneration
    05 (71.4)4 (57.1)5 (71.4)
    11 (14.3)-2 (28.6)
    21 (14.3)3 (42.9)-
    Nuclear polymorphism
    04 (57.1)4 (57.1)3 (42.9)
    13 (42.9)3 (42.9)4 (57.1)
    2---

    Values are presented as numbers and precentages (%).

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    Saudi Medical Journal: 44 (2)
    Saudi Medical Journal
    Vol. 44, Issue 2
    1 Feb 2023
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    Does the preoperative fasting period affect the liver in a distant organ model of renal ischaemia reperfusion?
    Yasemin Akcaalan, Ersin Gurkan Dumlu, Ebru Menekse, Mustafa Cem Yilmaz, Ezgi Erkilic, Betul Ogut, Aylin Sepıcı Dıncel
    Saudi Medical Journal Feb 2023, 44 (2) 171-177; DOI: 10.15537/smj.2023.44.2.20220582

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    Does the preoperative fasting period affect the liver in a distant organ model of renal ischaemia reperfusion?
    Yasemin Akcaalan, Ersin Gurkan Dumlu, Ebru Menekse, Mustafa Cem Yilmaz, Ezgi Erkilic, Betul Ogut, Aylin Sepıcı Dıncel
    Saudi Medical Journal Feb 2023, 44 (2) 171-177; DOI: 10.15537/smj.2023.44.2.20220582
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    Keywords

    • ERAS
    • ischaemia reperfusion
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