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Research ArticleOriginal Article
Open Access

Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines

Nouf A. ALkharashi
Saudi Medical Journal March 2023, 44 (3) 246-252; DOI: https://doi.org/10.15537/smj.2023.44.3.20220768
Nouf A. ALkharashi
From the Department of Home Eonomy, College of Education, Prince Sattam bin Abdulaziz University, Al-kharj, Kingdom of Saudi Arabia.
PhD
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    Figure 1

    - Cytotoxicity assessment by MTT assay in MCF-7 and HepG2 cell lines treated with varius concentrations of resveratrol for 24 hours. **p<0.01

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    Figure 2

    - The percentage of MCF-7 and HepG2 cells viability using a Trypan blue extraction assay for 24 hours exposure with different concentrations of resveratrol. **p<0.01

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    Figure 3

    - The mRNA levels of caspase-3, caspase-8, and caspase-9 in MCF-7 and HepG2 cells. **p<0.01

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    Figure 4

    - The mRNA levels of apoptotic markers (Bax, Bcl-2, and Bcl-xL) in MCF-7 and HepG2 cells. **p<0.01

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    Figure 5

    - The mRNA levels of apoptotic markers of p53 and p21 in MCF-7 and HepG2. **p<0.01

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    Table 1

    - The primer of genes involved in apoptosis and antiapoptotic genes.20-23

    Gene namesPrimers sequence
    Caspase-3F: 5′-GCTGGATGCCGTCTAGAGTC-3′
    R: 5′-ATGTGTGGATGATGCTGCCA-3′
    Caspase-8F: 5′-AGAAGAGGGTCATCCTGGGAGA-3′
    R: 5′-TCAGGACTTCCTTCAAGGCTGC-3′
    Caspase-9F: 5′-ATTGCACAGCACGTTCACAC-3′
    R: 5′-TATCCCATCCCAGGAAGGCA-3′
    BaxF: 5′-GAGCTAGGGTCAGAGGGTCA-3′
    R: 5′-CCCCGATTCATCTACCCTGC-3′
    Bcl-2F: 5′-ACCTACCCAGCCTCCGTTAT-3′
    R: 5′-GAACTGGGGGAGGATTGTGG-3′
    Bcl-XLF: 5′-CAGAGCTTTGAACAGGTAG-3′
    R: 5′-GCTCTCGGGTGCTGTATTG-3′
    p53F: 5′-GCTCTGACTGTACCACCATCC-3′
    F: 5′-CTCTCGGAACATCTCGAAGCG-3′
    p21F: 5′-CTCAGAGGAGGCGCCATG-3′
    R: 5′-GGGCGGATTAGGGCTTCC-3′
    GAPDHF: 5′-CGGAGTCAACGGATTTGGTC-3′
    R: 5′-AGCCTTCTCCATGGTCGTGA-3′
    Caspase: caspase protein, Bax: Bcl-2-associated X protein, Bcl-2: B-cell lymphoma 2, Bcl-XL: B-cell lymphoma-extra large, p53: Tumor protein P53, p21: Cyclin-dependent kinase inhibitor 1, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase
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Saudi Medical Journal: 44 (3)
Saudi Medical Journal
Vol. 44, Issue 3
1 Mar 2023
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Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines
Nouf A. ALkharashi
Saudi Medical Journal Mar 2023, 44 (3) 246-252; DOI: 10.15537/smj.2023.44.3.20220768

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Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines
Nouf A. ALkharashi
Saudi Medical Journal Mar 2023, 44 (3) 246-252; DOI: 10.15537/smj.2023.44.3.20220768
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Keywords

  • MTT
  • resveratrol
  • proliferation
  • MCF-7
  • HepG2 cell line

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© 2025 Saudi Medical Journal Saudi Medical Journal is copyright under the Berne Convention and the International Copyright Convention.  Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work. Electronic ISSN 1658-3175. Print ISSN 0379-5284.

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