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Research ArticleOriginal Article
Open Access

Elevated levels of CD56+ T Cells, CD16+ CD56+ T Cells, and CD56dim NK Cells in herpes simplex virus type 1 seropositive healthy individuals

Mazen Almehmadi, Mamdouh Allahyani, Abdulelah Aljuaid, Meshari A. Alsuwat and Mustafa Halawi
Saudi Medical Journal December 2024, 45 (12) 1312-1317; DOI: https://doi.org/10.15537/smj.2024.45.12.20240498
Mazen Almehmadi
From the Department of Clinical Laboratory Sciences (Almehmadi, Allahyani, Aljuaid, Alsuwat), College of Applied Medical Sciences, Taif University, Taif; and from the College of Nursing and Health Sciences (Halawi), Jazan University, Jazan, Kingdom of Saudi Arabia.
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Mamdouh Allahyani
From the Department of Clinical Laboratory Sciences (Almehmadi, Allahyani, Aljuaid, Alsuwat), College of Applied Medical Sciences, Taif University, Taif; and from the College of Nursing and Health Sciences (Halawi), Jazan University, Jazan, Kingdom of Saudi Arabia.
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Abdulelah Aljuaid
From the Department of Clinical Laboratory Sciences (Almehmadi, Allahyani, Aljuaid, Alsuwat), College of Applied Medical Sciences, Taif University, Taif; and from the College of Nursing and Health Sciences (Halawi), Jazan University, Jazan, Kingdom of Saudi Arabia.
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Meshari A. Alsuwat
From the Department of Clinical Laboratory Sciences (Almehmadi, Allahyani, Aljuaid, Alsuwat), College of Applied Medical Sciences, Taif University, Taif; and from the College of Nursing and Health Sciences (Halawi), Jazan University, Jazan, Kingdom of Saudi Arabia.
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Mustafa Halawi
From the Department of Clinical Laboratory Sciences (Almehmadi, Allahyani, Aljuaid, Alsuwat), College of Applied Medical Sciences, Taif University, Taif; and from the College of Nursing and Health Sciences (Halawi), Jazan University, Jazan, Kingdom of Saudi Arabia.
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    Figure 1

    - The density plots were extracted by FACs Diva software and showed the different levels between HSV-1 seropositive and seronegative participants. A is extracted from seronegative while B was from seropositive individual. The percentage for the gated populations is as follows; A density plot dim NK: 4%, bright NK: 0.5%, CD56+CD3+: 2.5%, and CD56-T-cells: 48.2%. B density plot dim NK: 9.2%, bright NK: 1.1%, CD56+CD3+: 8.4%, and CD56-T-cells: 47.5%. NK: natural killer cell, HSV-1: Herpes simplex virus type 1

  • Figure 2
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    Figure 2

    - Histograms were extracted by FACs diva software and showed the different levels between HSV-1 seropositive and seronegative participants in terms of CD16 expression. The percentage of expression was extracted by creating statics table, the mean was calculated and compared between the cell’s populations.

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    Table 1

    - Unpaired T-test was applied to compare the means between seronegative and seropositive individuals.

    VariablesHSV-1 
    Seronegative %Seropositive %P-value
    Bright NK1.61.80.67
    Dim NK4.69.20.04*
    NK11.6150.11
    CD56+ T-cells2.98.90.003*
    CD56- T-cells51.248.80.87
    • ↵* Indicates statistical significance set at p≤0.05. NK: natural killer cell, HSV-1: Herpes simplex virus type 1

    • View popup
    Table 2

    - UnpairedT-test was applied to compare the means between seronegative and seropositive individuals.

     HSV-1 
    VariablesSeronegative %Seropositive %P-value
    CD16+ Bright NK51560.67
    CD16+ Dim NK96970.89
    CD16+ NK91960.18
    CD16+ CD56+ T-cells62760.031*
    CD16+ CD56- T-cells4.15.40.09
    • ↵* Indicates statistical significance set at p≤0.05. Only CD56+ T-cells showed significant difference in terms of CD16 expression. The rest have higher levels but statistical insignificant. NK: natural killer cell, HSV-1: herpes simplex virus type 1

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Saudi Medical Journal: 45 (12)
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Elevated levels of CD56+ T Cells, CD16+ CD56+ T Cells, and CD56dim NK Cells in herpes simplex virus type 1 seropositive healthy individuals
Mazen Almehmadi, Mamdouh Allahyani, Abdulelah Aljuaid, Meshari A. Alsuwat, Mustafa Halawi
Saudi Medical Journal Dec 2024, 45 (12) 1312-1317; DOI: 10.15537/smj.2024.45.12.20240498

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Elevated levels of CD56+ T Cells, CD16+ CD56+ T Cells, and CD56dim NK Cells in herpes simplex virus type 1 seropositive healthy individuals
Mazen Almehmadi, Mamdouh Allahyani, Abdulelah Aljuaid, Meshari A. Alsuwat, Mustafa Halawi
Saudi Medical Journal Dec 2024, 45 (12) 1312-1317; DOI: 10.15537/smj.2024.45.12.20240498
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Keywords

  • CD56+
  • T-cells
  • HSV-1

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