Investigation of the effect of belinostat on MCF-7 breast cancer stem cells via the Wnt, Notch, and Hedgehog signaling pathway

Article Figures & Data

Download figure
Open in new tab
Download powerpoint
Figure 1
- The FACS analysis. A) The amount of MCF-7 total cell without belinostat. B) The amount of MCF-7 total cell with belinostat applied. C) Amount of CD44+/CD24- antibody treated MCF-7 stem cell without belinostat applied. D) Amount of CD44 +/CD24- antibody applied MCF-7 stem cells with belinostat applied. Q1: CD44+/CD24-, Q2: CD44+/CD24+, Q3: CD44-/CD24-, Q4: CD44-/24+
Download figure
Open in new tab
Download powerpoint
Figure 2
- Tumorsphere formation.
Download figure
Open in new tab
Download powerpoint
Figure 3
- Gene expression levels of Wnt, Hedgehog, Notch, and stem cell markers in MCF-7 cells.
Download figure
Open in new tab
Download powerpoint
Figure 4
- Gene expression levels of Wnt, Hedgehog, Notch, and stem cell markers in HEK-293 cells.
Download figure
Open in new tab
Download powerpoint
Figure 5
- Gene expression levels of Wnt, Hedgehog, Notch, and stem cell markers MCF-7 cells in MCF-7 CD44+/CD24-cancer stem cells.

Table 1

- Primer pairs nucleotide sequences.

Genes	Oligonucleotide sequences (5′-3′)	Bp	Refs.
WNT-1^**	GCGTCTGATACGCCAAAATC	244	41
WNT-1^**	GGATTCGATGGAACCTTCTG	244	41
LRP-6^**	AGGCACTTACTTCCCTGCAA	273	42
LRP-6^**	GGGCACAGGTTCTGAATCAT	273	42
APC^**	CAAAGACTCTGTATTGTTGGGAAAT	98	^*
APC^**	CATCAGGCTGTGAGTGAATGA	98	^*
TCF-7^**	GGACAACTACGGGAAGAAGAAG	95	^*
TCF-7^**	TCTCCGGGTAAGTACCGAAT	95	^*
FZD-8^**	TCTTGTCGCTCACATGGTTC	375	^*
FZD-8^**	TGTAGAGCACGGTGAACAGG	375	^*
LEF-1^**	GAAGCCTCAGCATGAACAGA	110	^*
LEF-1^**	CAACGACATTCGCTCTCATTTC	110	^*
NOTCH-1^†	CAATGTGGATGCCGCAGTTGTG’	125	43
NOTCH-1^†	CAGCACCTTGGCGGTCTCGTA	125	43
JAG-1^†	GTATCTGTCCACCTGGCTATG	120	^*
JAG-1^†	ACAGACACTGGAATCTGTTGAT	120	^*
SHH^{^‡}	CCGAGCGATTTAAGGAACTCA	133	^*
SHH^{^‡}	ATCACCGAGATGGCCAAAG	133	^*
PTCH^‡	GGGTGGCACAGTCAAGAACAG	108	44
PTCH^‡	TACCCCTTGAAGTGCTCGTACA	108	44
SMO^{^‡}	ATCGCTACCCTGCTGTTATTC	90	^*
SMO^{^‡}	GGGCACCATCCATGAACT	90	^*
GLI-1^{^‡}	GAGCTGGACATGCTGGTT	148	^*
GLI-1^{^‡}	ATTCAGGCTCACGCTTCTC	148	^*
MMP-1^{^§}	GCCTTCCAACTCTGGAGTAATG	111	^*
MMP-1^{^§}	AGGAGAGTTGTCCCGATGAT	111	^*
SOX^{^§}	GCCGAGTGGAAACTTTTGTCG	150	^*
SOX^{^§}	GGCAGCCTGTACTTATCCTTC	150	^*
NANOG^††	TTTGTGGGCCTGAAGAAAACT	116	^*
NANOG^††	AGGGCTGTCCTGAATAAGCAG	116	^*
OCT4^{^††}	CAAGGAGGCAAAGGTCTCGAA	193	^*
OCT4^{^††}	CGGCACCTGACACAGAAGA	193	^*
GAPDH^{^‡‡}	GGAGCGAGATCCCTCCAAAAT	197	^*
GAPDH^{^‡‡}	GGCTGTTGTCATACTTCTCAT	197	^*
CYPA^‡‡	TATCTGCACTGCCAAGACTGAGTG	124	^*
CYPA^‡‡	CTTCTTGCTGCTGGTCTTGCATTCC	124	^*