Hormone receptor status and survival of medullary breast cancer patients

Discussion

Breast cancer is one of the most prevalent cancers among women, but histological subtypes of breast cancer and especially MBC are seen rarely and1 also known for their favorable prognosis.4 The features of MBC such as histomorphologic structure and prognosis were discussed in some studies.8-12 In the present study, we described prognostic factors of recurrence and survival time in patients who were diagnosed with MBC. Women under 40 years of age are usually defined as young individuals and those under 35 are defined as very young in the literature.13,14 The patients with MBC are generally younger than other patients who had different types of breast cancer and patients who had breast cancer at earlier ages were associated with BRCA1-2 mutations.1,2 Median age of patients was 47 years old and the rate of younger patients (<35) was 10.4% in our study, similar to the findings of Park et al,9 which reported that median age of patients was 44 years old, and the rate of younger patients was 13.5%. Based on our results, the low incidence of MBC among very young women was consistent with the findings in the literature.1,2,7 According to previous studies, T2 tumors were identified as the most common and T1 tumors were determined as the least common type in the present study.7-11 Similar to previously mentioned studies, the lymph node involvement was not observed in the majority of our patients. Most of the patients (65.7%) had no lymph node involvement, while 22.9% of the patients with N1, 8.5% with N2, and 3% with N3 breast tumors were observed. In our study, we determined that this was reflected positively on the RFS in patients’ absence of lymph nodes, similar to the literature.8,9,15

Hormonal receptors, such as ER, PR, Her/neu-2 are predictive prognostic factors, and cornerstone of the treatment of patients with breast cancer. It is known that MBC was observed with the lowest frequency of ER, PR, Her/neu-2 expression based on previous studies.1,16 The ratio of high grade (grade 3) tumor was 71.1% in our study. The presence of LVI and high grade tumors, which were detected among featured more aggressive tumors, were identified in a large portion of our patients. It was observed that the presence of LVI was determined as an unfavorable prognostic factor for RFS. It was determined predominantly as a high grade tumor similar to other studies.1,17,18 Furthermore, 66.3% of patients in terms of HR, 72.7% of patients in terms of PR status, 79.8% of patients in terms of Her/neu-2 were evaluated as negative, similar to other studies.19,20 In this case, it was considered that these patients could benefit less from hormone suppression therapy.

Although our study showed that more tumors were identified as HR negative (90.47%) and triple negative (61.9%) in the group of patients aged <35 years old, Cao et al.21 reported that, among patients aged <40 years old, 50% were in the HR negative group and 44% were in the triple negative group.

In the present study, we found that PR expression rate was low (27.3%). This ratio was identified as high expression,21 although some of the cases were identified as low expression similar to our findings.18,20 Although studies including PR status associated with MBC are rare in the literature, Cao et al.21 determined that positivity of PR present an unfavorable risk factor on RFS patients with MBC. Rakha et al.22 reported that tumors with ER negative/PR positive status had more aggressive clinical outcomes than tumors with ER positive/PR negative status (p<0.01 for RFS, p<0.03 for OS) in all breast cancers. It has been demonstrated in recent studies that PR negative patients have no responder to complete response after neo-adjuvant chemotherapy in patients with local advanced breast cancer.23 We have determined that especially atypical histopathological patterns were significantly independent predictors of RFS (p<0.05). Similarly, Rakha et al24 obtained better survival rates in patients with the typical character of MBC when compared with patients who had atypical character, but they found that the difference was not statistically significant. In contrast, 4 of our 25 patients presented an atypical recurrence and a longer RFS. Typically, MBC is known to have prominent lymphocyte infiltration within and around the tumor sheets contrary to atypical MBC, which has less lymphocyte infiltration within tumor sheets. While lymphocyte infiltration in tumor tissue of typical MBC is denser, less lymphocyte infiltration is observed in atypical MBC.2,16,22,24 Although these features seem to be simple distinguishing factors of both types of MBC, pathologists sometimes fail to subtype MBC. Recently, a new terminology including both typical and atypical MBC, medullary-like carcinoma was emphasized.25 The recovery of some cancers spontaneously are more associated with cell-mediated immune response. Some researchers showed that some tumors such as melanoma can recover spontaneously with a high number of lymphocyte infiltration in tumor tissue. However, there are conflict results regarding its effects on the immune system of cancer pathogenesis in the literature.26,27 In our study, patients with atypical MBC present significant superior rates of RFS, which is not previously reported in the literature. We thought it was due to less tumor growth and angiogenesis with fewer lymphocytes, and mononuclear infiltration in atypical MBC. In the literature, it was shown that inflammatory cells may affect tumor growth with the release of angiogenetic and proteolytic enzymes and cofactors.28-30 Therefore, patients with typical MBC had poor prognosis in our outcomes similarly to Rakha et al24 outcomes. We demonstrated that negativity of PR and small size tumors had a statistically favorable prognostic effect on RFS and OS in MBC patients. The results of our study were similar to other studies in the literature.9,18,20 When we posed the question on why PR negative tumors had a longer survival effect, we found in the literature that triple negative tumors, especially PR negative tumors in MBC, were associated with BRCA1 mutation carriers. BRCA1 mutations were found in 12-13% of patients with a positive history of breast cancer in first degree relatives, those who were diagnosed less than 35 years of age and patients with breast cancer in synchronous or metachronous contralateral breast. Occurrence of breast cancer in BRCA1 mutation carriers also tend to lack PR and Her2/neu receptors, with heterogeneous tumor structures and has poor prognoses than cancers linked to BRCA2 mutation. According to some studies, BRCA1 mutation carriers were more likely to have ER-negative breast cancers.28,31,32 We found that PR negative rates were high (72.7%), and we observed that this situation does not represent a poor prognostic factor. Hence, we considered that the cancer of our patients was not associated with BRCA1 mutation.

Our study has several limitations. First, since this is a multicentre study, pathology samples were evaluated by different pathologists. Because of this issue, subtyping of MBC histopathology could have prevented an optimal diagnosis. It would have been preferable to have all samples be assessed by only one pathologist within certain norms Ridolfi’s17 or Pederson’s.33 Second, we did not conduct any further genetic evaluation in this study. If we could have carried out analysis for BRCA1-2, p-53 mutation, we would be able to discuss more accurate and more objective results regarding the clinical differences between BRCA1 and non-BRCA1 associated with tumors in MBC patients.

In conclusion, the patients with MBC were mostly diagnosed in early stages in Turkey. Although pathological characteristics (such as HR negativity, triple negative, high grade tumor) were determined as less favorable, patients with MBC have longer survival rates in our country. It is detected that good prognosis was observed in PR negative and small tumor size groups among patients with MBC. There may be a relationship between the PR negative tumor and BRCA1 mutation analysis. There is probably lower incidence of BRCA1 mutation in our region, because BRCA1 mutation probability is higher in PR negative MBC. Further studies are needed which would also include BRCA1-2 mutational analysis.