Article Figures & Data
Figures
- Figure 1
Micro-RNA-21 expression increased in hepatocellular carcinoma (HCC) patients. A) Quantitative polymerase chain reaction results comparing miR-21 levels in blood leukocyte among hepatitis B virus negative (HBV-) HCC patients, HBV positive HCC patients and healthy controls. Significant differences were calculated by non paired student-t test. B) Quantitative polymerase chain reaction results demonstrating the relationship between blood leukocyte miR-21 levels and HCC tissues miR-21 levels. C) Quantitative polymerase chain reaction results comparing the expression levels of miR-21 in HCC tissues and matched tumor-adjacent tissues. Significant differences were calculated by paired student-t test D) Quantitative polymerase chain reaction results comparing miR-21 levels between HBV negative and HBV positive HCC tissues. Significant differences were calculated by non paired student-t test.
- Figure 2
The receiver operating characteristic curve for blood leukocyte miR-21 level and serum AFP level in relation to the hepatocellular carcinoma patients.
- Figure 3
Kaplan-Meier curves for time to A) overall survival and B) disease-free survival of patients according to different miR-21 level.
- Figure 4
Association of miR-21 rs1292037 polymorphism with the miR-21 level in blood leukocyte of the hepatocellular carcinoma patients.
- Figure 5
Kaplan-Meier curves for time to A) overall survival and B) disease-free survival of patients according to different genetic polymorphism at genetic polymorphism at miR-21 rs1292037 SNP site.
- Figure 6
Kaplan-Meier curves for time to A) overall survival and B) disease-free survival of patients according to combined miR-21 rs1292037 polymorphism and hepatitis B virus infection status.
- Figure 7
Serum A) Alanine aminotransferase, B) aspirate aminotransferase, C) total bilirubin test, and D) direct bilirubin levels before and after partial hepatectomy of hepatocellular carcinoma patients with different genetic polymorphism at miR-21 rs1292037 polymorphism single nucleotide polymorphisms site. AA: miR-21 rs1292037 genotype is AA; AG/GG: miR-21 rs1292037 genotype is AG or GG. PRE: before hepatectomy AS 1 day: 1 day after hepatectomy; AS 3 day: 3 day after hepatectomy; AS 7 day: 7 day after hepatectomy.
- Figure 8
Micro-RNA-21 protect hepatocellular carcinoma cell injure and promote proliferation. A) Huh7 cells were exposed to simulated IRI environment or not, and then quantitative polymerase chain reaction were used to determine the miR-21 levels in both ischemic period and reperfusion period; B) Huh7 cells were transfected with miR-21 mimincs or miR-21 NC, following with simulated IRI exposure, and then intracellular ROS levels were measured by a fluorometry assay using intracellular oxidation of DCFH-DA; C) Huh7 cells were transfected with miR-21 mimincs or not, following with simulated IRI exposure or not, and then the cell proliferation were determined.
- Figure 9
Relative lucifer activity. A) The region of the human TNFα mRNA 3’UTR predicted to be targeted by miR-21. B) 293T cells were transiently co-transfected with luciferase reporter vectors for TNFα, and either miR-21 mimics, inhibitor or negative control. Luciferase activities were normalized to the activity of Renilla luciferase. C) Huh7 cells were transfected with miR-21 mimincs, inhibitor or miR-21 NC, following with simulated ischemia-reperfusion injury (IRI) exposure, and then intracellular IL-12A mRNA expression were determined by qRT-PCR assay. D) Huh7 cells were transfected with miR-21 mimincs or miR-21 negative control, together with IL-12 treatment (10ng/mL) or not, following with simulated IRI exposure, and then the cell proliferation were determined
- Figure 10
MiR-21 rs1292037 polymorphism was associated with the miR-21 functions in hepatocellular carcinoma cell (HCC) cells by regulating IL-12A expressions. A) Primary HCC cells were isolated from 22 cases of rs1292037 AA and 57 cases of AG/GG and were incubated for 24 h. The cells were harvest and the miR-21 expressions were assayed by quantitative reverse-transcript polymerase chain reaction (qRT-PCR). B) Cells in (A) were simulated IRI exposure, and then intracellular receiver operating characteristic levels were measured by a fluorometry assay using intracellular oxidation of DCFH-DA. C) Cells in (A) were simulated IRI exposure or not, and then the cell proliferation were determined. D) Cells in (A) were harvest and the IL-12A expressions were assayed by qRT-PCR. E) quantitative polymerase chain reaction results demonstrating the relationship between miR-21 rs1292037 polymorphism and IL-12A expressions in blood leukocyte of the HCC patients.
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